The Early Stages in the Interaction Between Mycorrhizal Fungi and Plants

菌根真菌与植物相互作用的早期阶段

• 批准号: 9630842
• 负责人: Ann Hirsch
• 金额: $ 5.23万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-15 至 1998-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630842&HistoricalAwards=false
• 关键词: Early; Stages; Interaction; Between; Mycorrhizal

9630842 Hirsch The Sinorhizobium meliloti-alfalfa symbiosis, which leads to the formation of novel structure--the root nodule in which atmospheric nitrogen is reduced to ammonia--has been well studied. On the other hand, the mycorrhizal symbiosis, which enables plants to grow in phosphorous-deficient soil, is less well studied. Chemical signals produced by both the plant and the bacterium are involved in establishing the interaction between the two organisms. Dr. Hirsch and her colleagues have determined that VAM-fungi induce the expression of at least two plant genes in mycorrhizal roots that are usually triggered by inoculation with rhizobia. Alfalfa plants will be studied for their early responses to the VAM fungi. The roots will be inoculated with the spores of the fungi, the progress of infection by fungal hyphae will be followed, and the timing and patterns of expression of selected plant genes will be analyzed. This award supports feasibility of using the expression of these early nodulin genes as molecular indicators for developing a bioassay to detect a putative Myc factor, i.e., a chemical signal that induces the expression of specific plant genes in VA-mycorrhizal roots, and is involved in establishing this important symbiosis.

9630842 Hirsch苜蓿中华根瘤菌与苜蓿共生，导致形成新结构--大气氮还原为氨的根瘤--已得到充分研究。 另一方面，使植物能够在缺磷土壤中生长的菌根共生关系研究较少。 由植物和细菌产生的化学信号参与建立两种生物体之间的相互作用。 Hirsch博士和她的同事们已经确定VAM真菌诱导菌根根中至少两种植物基因的表达，这些基因通常是由接种根瘤菌引发的。 将研究alcohol植物对VAM真菌的早期反应。 将用真菌的孢子接种根，将跟踪真菌菌丝感染的进展，并分析所选植物基因的表达时间和模式。 该奖项支持了使用这些早期麦谷蛋白基因的表达作为分子指标用于开发生物测定以检测推定的Myc因子的可行性，即，一种化学信号，诱导VA菌根根中特定植物基因的表达，并参与建立这种重要的共生关系。