Isolation of a Receptor for a Fungal-Wall-Derived Eliitor of Phytoalexins

真菌壁衍生的植物抗毒素Eliitor受体的分离

• 批准号: 8704022
• 负责人: Michael Hahn
• 金额: $ 8万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1990-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8704022&HistoricalAwards=false
• 关键词: Isolation; Receptor; Fungal; Wall; Derived

Isolation of a Receptor for a Fungal-Wall-Derived Elicitor of Phytoalexins: The long term goal of the research described in this proposal is the isolation and characterization of a hepta-B-glucoside receptor from soybean tissue. Fungal-derived B-glucans of the type from which the hepta-B-glucoside was isolated are active as inducers or enhancers of a variety of defense mechanisms in both plants and animals. No receptor for oligosaccharide(s) of this type has been isolated from any source. The only biologically active B-glucan fragment that has been purified to homogeneity and structurally characterized is a hepta-B-glucoside that is active at nanomolar concentrations in stimulating phytoalexin (antibiotic) accumulation in soybean tissue. The specific goals for the initial period of funding are to identify the most suitable soybean tissue from which to isolate the receptor for the active hepta-B-glucoside elicitor, to prepare various derivatives of the hepta-B-glucoside elicitor and to compare their biological activity with that of the native elicitor. These derivatives will be used to prepare (i) radiolabeled elicitor for ligand-binding studies, (ii) photo-affinity labeling reagents for receptor localization and identification studies, and (iii) affinity chromatography matrices for receptor purification. Time permitting, the elicitor derivatives will be used to initiate studies to identify putative receptors in plant extracts. Phytoalexin accumulation is believed to play an important role in plant disease resistance and also is an excellent model system for studying the regulation of gene expression in plants. Purification of the hepta-B-glucoside receptor is necessary before studies can be undertaken to identify the role the receptor(s) play in the signal pathway that results in altered gene expression leading to phytoalexin accumulation in affected plant cells. The research should increase our understanding of how plants perceive invasive microorganisms and how the recognition leads to activation of specific plant defenses.

真菌壁衍生的激发子的受体的分离 植物抗毒素：所述研究的长期目标 在这个提议中， 来自大豆组织七-B-葡萄糖苷受体。 真菌衍生的 七-B-葡糖苷来源类型的B-葡聚糖 分离的化合物作为多种免疫调节剂的诱导剂或增强剂是有活性的， 植物和动物的防御机制。 没有受体 这种类型的寡糖已经从任何 源头 唯一具有生物活性的β-葡聚糖片段， 已纯化至均质并进行结构表征 是一种七-B-葡糖苷，在纳摩尔浓度下具有活性 植物抗毒素（抗生素）浓度 大豆组织中的积累。 的具体目标 资金的初始阶段是确定最合适的 大豆组织，从该组织中分离出针对 活性七-B-葡萄糖苷诱导剂， 七-B-葡萄糖苷诱导剂的衍生物，并比较 它们的生物活性与天然激发子的生物活性相同。 这些衍生物将用于制备（i）放射性标记的 用于配体结合研究的激发子，（ii）光亲和性 用于受体定位和鉴定的标记试剂 研究，和（iii）亲和层析基质， 受体纯化 时间允许的话， 衍生物将用于启动研究，以确定 植物提取物中的假定受体。 植物抗毒素的积累被认为是重要的 在植物抗病中的作用，也是一个很好的模型 研究基因表达调控的系统， 植物 七-B-葡糖苷受体的纯化是 在进行研究以确定其作用之前， 受体在信号通路中起作用， 改变基因表达导致植物抗毒素积累， 影响植物细胞。 这项研究应该增加我们的 了解植物如何感知入侵微生物， 识别如何导致特定植物的激活 的防御合作.