Constitutive Potassium Transport Systems in E. coli

大肠杆菌中的组成性钾转运系统

• 批准号: 8704059
• 负责人: Wolfgang Epstein
• 金额: $ 19.5万
• 依托单位: University of Chicago
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1990-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8704059&HistoricalAwards=false
• 关键词: Constitutive; Potassium; Transport; Systems; E.

Constitutive Potassium Transport Systems in E. coli: Several constitutive potassium transport systems of the Gram- negative bacterium,-Escherichia coli, will be analyzed. The analysis of the moderate affinity Trk system will include determination of the four genes implicated in that stystem but not yet sequenced. The subcellular location of the gene products and interactions among these proteins will be defined. Residual potassium transport activity in mutants lacking Trk will be the subject of genetic analysis. Major systems of potassium efflux will be identified by mutational analysis. After the systems are identified, their protein components, kinetics and regulation will be deterimined. The results of this research should increase our understanding of the mechanisms and regulation of all ways that potassium homeostasis is maintained in E. coli. It is not unreasonable to assume that the patterns, mechanisms and principles discovered in bacteria will be useful in understanding similarities in other cells with walls such as plant cells. Moreover, because the high affinity Kdp transport system is homologous to transport ATPases of higher eucaryotes the results should be instructive for the bioenergetics of mammalian systems.

大肠杆菌的组成钾运输系统：革兰氏阴性细菌-大肠杆菌的几个组成钾运输系统将被分析。中等亲和力Trk系统的分析将包括确定与该系统有关但尚未测序的四个基因。基因产物的亚细胞位置和这些蛋白质之间的相互作用将被定义。缺乏Trk的突变体的剩余钾转运活性将是遗传分析的主题。钾外排的主要系统将通过突变分析来确定。在系统被识别后，它们的蛋白质成分、动力学和调控将被确定。这项研究的结果将增加我们对大肠杆菌中钾稳态维持的机制和调控的理解。假设在细菌中发现的模式、机制和原理将有助于理解其他有壁细胞（如植物细胞）的相似性，这并非不合理。此外，由于高亲和力的Kdp转运系统与高等真核生物的转运atp酶同源，因此该结果对哺乳动物系统的生物能量学具有指导意义。