Acquisition of a Multiwire Area Detector System for Macromolecular Crystallographic Studies

将获得一个多丝区域探测器系统，作为一种快速记录蛋白质和核酸单晶X射线衍射数据的方法。该系统包括多丝区域探测器腔、全圆测角仪、石墨单色仪、旋转阳极X射线发生器的安装改造以及用于数据采集和处理的VAX II站的400兆磁盘驱动器。该设备将用于大分子结构的结晶学分析，作为与植物科学、分子生物学、细胞生物学生物技术相关的当前生物学研究问题的一部分。这些项目包括：(1)蛋白质延伸因子复合体和相关的G蛋白，(2)核酸结合蛋白和蛋白质-核酸复合体，(3)单糖和多糖加工蛋白，(4)糖蛋白，(5)血清运输蛋白和(6)农业相关蛋白。面积探测器将允许对脆弱的晶体进行检查，并促进更快速的数据收集，并允许对重大生物学问题进行分子水平的理解。

A multiwire area detector system will be acquired to serve as a rapid method for recording X-ray diffraction data from single crystals of proteins and nucleic acids. The system includes a multiwire area detector chamber, a full-circle goniometer, graphite monochromator, mounting modifications for a rotating- anode X-ray generator and a 400 megabyte disk drive for a VAX II Station for data acquisition and processing. The equipment will be used for crystallographic analysis of macromolecular structures as part of current biological research problems relevant to plant science, molecular biology, cell biology biotechnology. The projects include: (1) protein elongation factor complexes and related G proteins, (2) nucleic acid-binding proteins and protein-nucleic acid complexes, (3) mono- and polysaccharide processing proteins, (4) glycoproteins, (5) serum transport proteins and (6) agriculturally-related proteins. The area detector will allow fragile crystals to be examined as well as facilitate more rapid data collection, and permit a molecular level of understanding for significant biological questions.