Acquisition of a Multiwire Area Detector System for Macromolecular Crystallographic Studies

获取用于大分子晶体学研究的多线区域检测器系统

• 批准号: 8704438
• 负责人: Frances Jurnak
• 金额: $ 14万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8704438&HistoricalAwards=false
• 关键词: Acquisition; Multiwire; Area; Detector; System

A multiwire area detector system will be acquired to serve as a rapid method for recording X-ray diffraction data from single crystals of proteins and nucleic acids. The system includes a multiwire area detector chamber, a full-circle goniometer, graphite monochromator, mounting modifications for a rotating- anode X-ray generator and a 400 megabyte disk drive for a VAX II Station for data acquisition and processing. The equipment will be used for crystallographic analysis of macromolecular structures as part of current biological research problems relevant to plant science, molecular biology, cell biology biotechnology. The projects include: (1) protein elongation factor complexes and related G proteins, (2) nucleic acid-binding proteins and protein-nucleic acid complexes, (3) mono- and polysaccharide processing proteins, (4) glycoproteins, (5) serum transport proteins and (6) agriculturally-related proteins. The area detector will allow fragile crystals to be examined as well as facilitate more rapid data collection, and permit a molecular level of understanding for significant biological questions.

将获得一个多线区域探测器系统， 一种快速记录单晶X射线衍射数据的方法 蛋白质和核酸的晶体。 该系统包括 多线面积探测器室，全圆测角仪， 石墨单色器，安装改造的旋转- 阳极X射线发生器和VAX II的400兆磁盘驱动器 数据采集和处理站。 这些设备将 用于大分子的晶体学分析 结构作为当前生物学研究问题的一部分 与植物科学、分子生物学、细胞生物学相关 生物技术. 项目包括：（1）蛋白质延伸 因子复合物和相关G蛋白，（2）核酸结合 蛋白质和蛋白质-核酸复合物，（3）单-和 多糖加工蛋白，（4）糖蛋白，（5）血清 运输蛋白和（6）农业相关蛋白。 的 区域探测器将允许脆弱的晶体进行检查，以及 以促进更快速的数据收集，并允许分子 对重大生物学问题的理解水平。