Preparation and Crystallization of EF-Tu-GTP-Phe-tRNA and EF-Tu-GMP.PNP for X-Ray Diffraction Studies

用于 X 射线衍射研究的 EF-Tu-GTP-Phe-tRNA 和 EF-Tu-GMP.PNP 的制备和结晶

• 批准号: 8708465
• 负责人: Frances Jurnak
• 金额: $ 5万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-11-01 至 1990-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8708465&HistoricalAwards=false
• 关键词: Preparation; Crystallization; EF; Tu; GTP

Protein elongation factors play a major role in ribosomal protein synthesis. Dr. Jurnak has used both biochemical and X-ray diffraction analyses of elongation factor complexes in her research. Her main goal is to understand the molecular details of each step of the elongation cycle with a special focus on the structural basis for the activity of elongation factor EF-Tu. She will initiate new research projects which focus directly on the macromolecular crystallization problem itself. Dr. Jurnak will explore the feasibility of preparing large quantities of the purified complexes and to crystallizing each in a form suitable for X-ray diffraction analyses. She will also learn quasi-elastic light scattering (QELS) methods; a techique which will help to elucidate key macromolecular properties that affect the crystallization process. These complexes are of considerable biological interest. The Career Advancement Award offers her the opportunity to open up new crystallographic projects and to demonstrate the usefulness of the QELS technique for general applicability to macromolecular crystallization.

蛋白质延伸因子在核糖体中起主要作用。 蛋白质合成。 尤尔纳克博士使用了生物化学和 中延伸因子复合物的X射线衍射分析 她的研究。 她的主要目标是了解 拉伸周期的每个步骤的详细信息， 着重探讨了伸长活性的结构基础 因子EF-Tu。 她将启动新的研究项目， 直接关注大分子结晶问题 本身 尤尔纳克博士将探讨准备大型 纯化的复合物的量，并结晶每一个 其形式适合于X射线衍射分析。 她将 还学习准弹性光散射（QELS）方法; a 有助于阐明关键大分子技术 影响结晶过程的性质。 这些 配合物具有相当大的生物学意义。 职业发展奖为她提供了机会， 开辟新的晶体学项目， QELS技术的普遍适用性， 大分子结晶