Regulation of Chlorophyll Synthesis in Plant Cells

植物细胞中叶绿素合成的调控：将采用生物化学和遗传学的方法研究衣藻中δ -氨基乙酰丙酸的合成调控，δ -氨基乙酰丙酸是叶绿素卟啉环合成的第一个承诺化合物。将谷氨酸与其转移RNA连接起来的酶已被纯化。该途径的其余两种酶将被纯化，这三种酶的基因将被克隆并测序。了解δ -氨基乙酰丙酸的生物合成及其调控对了解植物细胞中叶绿素生物合成和光合膜组装的调控至关重要。由于已知的衣藻与高等植物的相似性，这些知识和基因可以转移，从而可用于基因工程，以提高植物的生长效率

Regulation of Chlorophyll Synthesis in Plant Cells: Biochemical and genetic approaches will be used to study in Chlamydomonas the regulation of synthesis of delta-aminolevulinic acid, the first committed compound in synthesis of the porphyrin ring of chlorophyll. The enzyme that ligates glutamate to its transfer RNA has been purified. The remaining two enzymes of the pathway will be purified and the genes for all three enzymes will be cloned and sequenced. Understanding the biosynthesis of delta-aminolevulinic acid and its regulation is essential for the understanding of the regulation of chlorophyll biosynthesis and the assembly of the photosynthetic membranes in plant cells. Because of the known similarities between Chlamydomonas and higher plants not only the knowledge but also the genes may be transferable and thus available to genetic engineering for increasing plant growth efficiency