Genetic Regulation of Chlorophyll Synthesis in Higher Plants

植物光形态建成是一个复杂的发育过程， 控制至少三种不同的光感受器， 细胞核和细胞器中的差异基因表达 基因组 在这个过程中的一个特点“问候”的地方。 我们感兴趣的是了解生物化学和分子 高等植物叶绿素形成的遗传控制。 我们有 鉴定的cDNA和/或克隆的基因组序列编码三种 叶绿素生物合成途径的酶。 这些酶 参与该途径或者作为调节（例如，NADPH- 原叶绿素氧化还原酶（PCR）或非限速步骤 (e.g., ALA-脱氨酶（ALAD）、PBG脱氨酶（PBGD）和 在光敏色素介导的光调节下， 阳性方式（ALAD）、阴性方式（PCR）或 不受光影响（PBGD）。 我们建议继续我们的 结构、复杂性和组织的表征 编码这三种叶绿体蛋白质的基因家族。 家庭成员个体的表达特点 将分析光、质体的范围 分化和细胞类型。 因为大量证据表明 支持了四吡咯双途径的存在 在高等植物的形成存在，我们将确定是否 这些基因家族的单个成员产生蛋白质 定位于不同的亚细胞区室（叶绿体 如果是这样，基因家族的成员是否 产生蛋白质， 不同的地方有不同的监管计划。 绿色植物产生叶绿素， 环境，并作为其正常发展的一部分， 流程. 叶绿素生物合成的控制是复杂的， 不太了解。 这是一项旨在阐明 控制。*

Plant photomorphogenesis is a complex developmental process under the control of at least three distinct photoreceptors and involving differential gene expression in both nuclear and organellar genomes. During this process a characteristic "greeting" of place. We are interested in understanding the biochemical and molecular genetic control of chlorophyll formation in higher plants. We have identified cDNAs and/or cloned genomic sequences encoding three enzymes of the chlorophyll biosynthetic pathway. These enzymes participate in this pathway either as regulatory (e.g., NADPH- protochlorophyllide oxidoreductase (PCR) or non-rate-limiting steps (e.g., ALA-dehydratase (ALAD), PBG deaminase (PBGD) and are thought to be under phytochrome-mediated photoregulation either in a positive manner (ALAD), a negative manner (PCR), or to be unaffected by light (PBGD). We propose to continue our characterization of the structure, complexity, and organization of the gene families that encode these three chloroplast proteins. The expression characteristics of the individual family members will be analyzed with respect to light, extent of plastid differentiation and cell type. Since substantial evidence supporting the presence of a dual pathway for tetrapyrrole formation in higher plants exists, we will determine whether individual members of these gene families give rise to proteins localized in different subcellular compartments (chloroplastic versus cytosolic) and if so, whether members of a gene family giving rise to proteins compartmentalized in different subcellular locations are under different regulatory programs. Green plants produce chlorophyll both in response to light in their environment, and as part of their own normal developmental processes. The control of chlorophyll biosynthesis is complex, but poorly understood. This is a proposal to elucidate that control.***