RUI: Acquisition of a DNA Synthesizer System

首席研究员已申请购买资金 DNA合成器系统 设备将由以下人员共享： 五名使用重组DNA技术的研究人员， 研究项目迫切需要现场寡核苷酸 合成能力，以产生用于DNA合成的引物， 体外诱变 该合成器最初将用于 这些项目涉及到人类的分子遗传学， 果蝇肌球蛋白重链基因; 哺乳动物红系细胞; RNA病毒基因调控 表达;编码小核RNA的基因的表达; 并启动E.杆菌 的 仪器将直接受益于圣地亚哥州 大学的技术增加，学生熟悉 与当前的技术。

The principal investigator has requested funds for the purchase of a DNA Synthesizer System. The equipment will be shared by five investigators who use recombinant DNA techniques and whose research programs are in critical need of on-site oligonucleotide synthesis capability to produce primers for DNA synthesis and in vitro mutagenesis. The synthesizer will be utilized initially in projects that are concerned with the molecular genetics of the Drosophila myosin heavy chain gene; enzyme degradation in mammalian erythroid cells; regulation of RNA virus gene expression; expression of genes encoding the small nuclear RNAs; and initiation of DNA replication in E. coli. The instrumentation will directly benefit the San Diego State University's techniques increases and students gain familiarity with current technology.