Genetic Analysis of Bacteroides Polysaccharide Utilization Systems

拟杆菌多糖利用系统的遗传分析

• 批准号: 8706706
• 负责人: Abigail Salyers
• 金额: $ 5万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-10-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8706706&HistoricalAwards=false
• 关键词: Genetic; Analysis; Bacteroides; Polysaccharide; Utilization

This is a Career Advancement Award which allows Dr. Salyers to develop new genetic tools for understanding metabolic strategies of Bacteroides bacteria. Specific aims are to (l) Construct a derivative of an insertion sequence that could be used to detect Bacterioides genes on the basis of their regulatory properties, (2) Use a fusion of the Emr gene to the chondroitin lyase II promoter to select for mutations that lead to constrituitive expression of genes in the chondroitin sulfate breakdown system, (3) construct a cosmid shuttle vector that can be mobilized into Bacteroides and is maintained in Bacteroides as a plasmid, and (4) Obtain and characterize transposon mutants in the alpha-galactoside utilization system of B. ovatus, and to place them in complementation groups using a library of B. ovatus DNA cloned in the cosmid shuttle vector.

这是一项职业进步奖，使 Salyers 博士能够开发新的遗传工具来了解拟杆菌的代谢策略。 具体目标是 (l) 构建可用于根据调节特性检测拟杆菌基因的插入序列的衍生物，(2) 使用 Emr 基因与软骨素裂解酶 II 启动子的融合来选择导致硫酸软骨素分解系统中基因组成型表达的突变，(3) 构建可动员到其中的粘粒穿梭载体 拟杆菌并作为质粒维持在拟杆菌中，并且(4)获得并表征卵形拟杆菌的α-半乳糖苷利用系统中的转座子突变体，并使用克隆在粘粒穿梭载体中的卵形拟杆菌DNA文库将它们置于互补组中。