Biosynthesis of Thyrotropin Releasing Hormone

促甲状腺激素释放激素（TRH）在甲状腺功能亢进中起着重要作用。 上丘脑垂体甲状腺轴 尽管近二十年来 工作，几乎没有什么是已知的TRH的调节 生产 古德曼博士和合作者建议使用一种 最近分离的TRH cDNA和TRH产生细胞系， 描述TRH的因素和机制 生物合成 他将使用完整的动物来比较TRH样 在离散的大脑区域和大鼠细胞系中的mRNA，以确定 影响TRH生物合成和分泌的药物。 他还将 测试TRH前体是否可以产生肽， TRH影响垂体功能。 最后，他将使用 TRH产生细胞系，以鉴定TRH的组分 基因是重要的调控和组织特异性 表情 阐明控制TRH的因素和机制 生物合成和分泌会大大增加我们的 了解大脑如何调节内分泌功能。

Thyrotropin releasing hormone (TRH) plays a central role in the hopothalamic-pituitary-thyroid axis. Despite nearly twenty years of work, virtually nothing is known about the regulation of TRH production. Dr. Goodman and collaborators propose to use a recently isolated TRH cDNA and TRH-producing cell line to characterize the factors and mechanisms that underlie TRH biosynthesis. He will use intact animals to compare TRH-like mRNAs in discrete brain regions and a rat cell line to identify agents that affect TRH biosynthesis and secretion. He will also test whether the TRH precursor can generate peptides in addition to TRH that affect pituitary function. Finally, he will use the TRH-producing cell line to identify the components of the TRH gene that are important for regulated and tissue-specific expression. Elucidation of the factors and mechanisms that control TRH biosynthesis and secretion would greatly increase our understanding of how the brain regulates endocrine function.