Postdoctoral Research Fellowships in Plant Biology

植物生物学博士后研究奖学金

• 批准号: 8710687
• 负责人: Roger Innes
• 金额: $ 7.95万
• 依托单位: Individual Award
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-12-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8710687&HistoricalAwards=false
• 关键词: Postdoctoral; Research; Fellowships; Plant; Biology

Specific disease resistance is manifested by a hypersensitive response (HR) to the pathogen that includes localized necrosis, production of phytoalexins, and changes in cell wall structure. Specific R/A mediated HR's often differ phenotypically, even among congenic lines of plants. This observation suggests that an R/A gene interaction does not simply trigger a universal plant defense response. Instead, the defense response may, in part, be uniquely specified by the interactive R/A gene pair. This hypothesis will be tested by comparing, at the molecular genetic level, two specific R/A gene interactions in the Capsicum annuum/Xanthomonas campestris pv. vesicatoria interaction (bacterial leaf spot disease of green pepper). As part of this comparison the specific R genes will be identified and cloned. Once a specific resistance gene is cloned many experiments can be done that will elucidate the mechanisms of specific disease resistance. If a specific resistance gene can be cloned and characterized, a major breaktrhough will occur in our understanding and ability to control disease in plants. But even if a specific gene cannot be identified, a detailed comparison of two different R/A gene interactions at the molecular genetic level will provide insight into how specific resistance is conferred.

特异性抗病表现为对病原菌的超敏反应(HR)，包括局部坏死、植物抗毒素的产生和细胞壁结构的改变。特异的R/A介导的HR往往表现不同，即使在植物的同源品系之间也是如此。这一观察表明，R/A基因的相互作用并不简单地触发普遍的植物防御反应。相反，防御反应可能在一定程度上是由交互式R/A基因对唯一指定的。这一假说将通过在分子遗传水平上比较辣椒/野油菜黄单胞菌中两个特定的R/A基因相互作用来验证。白粉菌互作(青椒细菌性叶斑病)。作为比较的一部分，特定的R基因将被识别和克隆。一旦克隆了特定的抗病基因，就可以进行许多实验来阐明特定抗病的机制。如果一个特定的抗性基因能够被克隆和鉴定，我们对植物病害的理解和控制能力将发生重大突破。但是，即使不能识别一个特定的基因，在分子遗传水平上对两个不同的R/A基因相互作用的详细比较将有助于深入了解特定抗性是如何产生的。