Comparative Analysis of Legume Genome Evolution

豆科植物基因组进化的比较分析

• 批准号: 0321664
• 负责人: Roger Innes
• 金额: --
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0321664&HistoricalAwards=false
• 关键词: Comparative; Analysis; Legume; Genome; Evolution

In the U.S., soybean (Glycine max) is second only to corn in total acres planted and is a primary source of proteins and oils for both animal and human consumption. A major feature of the soybean genome is a duplication event estimated to have occurred 9 million years ago. Similar polyploidization events are believed to have occurred multiple times in the evolutionary history of most, and perhaps all, flowering plants, and have lead to dramatic changes in genome structure. Major questions regarding the process of genome restructuring following polyploidization remain to be answered. Comparative genomic analyses will be used to identify patterns of gene rearrangement associated with both old (9 mya) and recent (50 kya) polyploidy events in the Glycine genus. Specifically, an approximately 1 megabasepair interval from each of six legume taxa will be sequenced, as will the duplicated regions in the polyploid members (12 intervals in total). These taxa include two soybean cultivars, the closest true diploid relative of soybean Teramnus labialus and a recent polyploidy relative, Glycine tomentella G1188, that has twice as many chromosomes as soybean. The genomic interval to be sequenced contains several commercially important disease resistance (R) genes in soybean, and contains both rapidly rearranging and relatively stable chromosomal blocks. These analyses will address fundamental questions regarding genome evolution on both short (100,000 years) and long (50 million years) time scales, as well as questions regarding the evolution of R genes. This sequence collection will also facilitate identification of conserved promoter elements and non-coding RNAs, and well as commercially useful R genes. All sequence data, physical maps, and analyses will be made publicly available through a project web-site. This project will also generate new public software for phylogenetic analysis of genome rearrangements, and bacterial artificial chromosome (BAC) libraries of 5 legume species.The project will be linked to the educational and non-science community via direct collaborations with two hands-on science museums in Bloomington, Indiana and Blacksburg, Virginia, and by two high school outreach programs at the University of Minnesota and Cornell University. Hands-on activity kits will be developed that will be used for public events at museums, and more importantly in K-12 classrooms. Classroom use will be promoted through a series of teacher training workshops hosted at each museum. All kits will be specifically designed to address state academic standards in science and will teach principles in plant biology and evolution.

在美国，大豆（Glycinemax）种植面积仅次于玉米，是动物和人类消费的蛋白质和油的主要来源。 大豆基因组的一个主要特征是估计发生在900万年前的复制事件。类似的多倍化事件被认为在大多数甚至所有开花植物的进化史中发生过多次，并导致了基因组结构的巨大变化。关于多倍体化后基因组重组过程的主要问题仍有待回答。比较基因组分析将被用来确定模式的基因重排与老（9百万年）和最近（50千）的多倍体事件在大豆属。 具体地说，将对来自六个豆科植物分类群中的每一个的大约1兆碱基对间隔进行测序，多倍体成员中的重复区域也是如此（总共12个间隔）。 这些分类群包括两个大豆品种，最接近的真正的二倍体相对大豆Teramnus labialus和最近的多倍体相对，Glycine tomentella G1188，有两倍的大豆染色体。 待测序的基因组区间包含大豆中几个商业上重要的抗病（R）基因，并且包含快速重排和相对稳定的染色体块。 这些分析将解决有关基因组进化的基本问题，无论是短（10万年）和长（5000万年）的时间尺度，以及有关R基因的进化问题。该序列收集还将有助于鉴定保守的启动子元件和非编码RNA，以及商业上有用的R基因。 所有序列数据、物理图和分析将通过项目网站公开提供。 该项目还将产生用于基因组重排的系统发育分析的新的公共软件和5种豆科植物的细菌人工染色体（BAC）文库。该项目将通过与印第安纳州的布卢明顿和弗吉尼亚州的布莱克斯堡的两个动手科学博物馆以及明尼苏达大学和康奈尔大学的两个高中外展项目的直接合作，与教育和非科学界联系起来。 将开发实践活动套件，用于博物馆的公共活动，更重要的是用于K-12教室。 将通过在每个博物馆举办的一系列教师培训讲习班促进课堂使用。 所有工具包将专门设计，以解决国家学术标准的科学，并将教授植物生物学和进化的原则。