Studies of Marginal Band Formation and Function in Erythrocytes

红细胞边缘带形成和功能的研究

• 批准号: 8711810
• 负责人: William Cohen
• 金额: $ 23.2万
• 依托单位: CUNY Hunter College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8711810&HistoricalAwards=false
• 关键词: Studies; Marginal; Band; Formation; Function

Continuous circumferential bundles of microtubules, known as marginal bands (MBs), are prominent cytoskeletal components in all non- mammalian vertebrate erythrocytes and a wide range of other blood cell types. In previous work the investigator has shown that MBs of mature cells can maintain erythrocyte shape against deformation by external forces. However, the function of the MB during erythrocyte morphogenesis from sphere to flattened ellipse remains unknown. In the proposed work the principal investigator will test a working hypothesis for the relationship between the state of MB assembly nd stage of cellular morphogenesis, in which pointed cells constitute intermediate stages. The tests include recording of the precise morphological sequence by phase contrast/video microscopy, immunofluorescence localization of microtubules and centriole-containing organizing centers, and analysis of microtubule polarity at an early critical stage. A related study on chick bone marrow erythroblasts in culture will determine whether cells of pointed morphology are a universal intermediate stage. The proposal also includes continued studies on the structure and composition of marginal bands isolated from dogfish erythrocytes by improved procedures, and of marginal band proteins obtained by selective solubilization from cytoskeletons. These studies will enhance our understanding of the mechanisms involved in the biogenesis and function of microtubule arrays. They will also provide insight into cytoskeletal function during cell differentiation, a significant issue in cell biology research.

微管的连续圆周束，称为 边缘带（MB）是细胞骨架的主要成分， 所有非哺乳动物脊椎动物红细胞和广泛的 其他类型的血细胞 在以前的工作中，研究人员 表明成熟细胞的MB可以维持红细胞的形状， 抵抗外力的变形。 然而，MB在红细胞中的功能 从球形到扁平椭圆形的形态发生仍然未知。 在拟议的工作中，主要研究者将测试一个 MB状态之间关系的工作假设 组装和阶段的细胞形态发生，其中指出 细胞构成中间阶段。 述测试包括 按相位记录精确的形态序列 对比/视频显微镜，免疫荧光定位， 微管和含中心粒的组织中心， 在早期关键阶段分析微管极性。 一 鸡骨髓成红细胞体外培养的相关研究 确定尖形形态的细胞是否是通用的 中间阶段 该建议还包括继续研究 边缘带的结构和组成， 用改良的方法对角鲨红细胞进行边缘带分析 从细胞骨架中选择性溶解而获得的蛋白质。 这些研究将增进我们对 微管的生物发生和功能机制 阵 他们也将提供深入了解细胞骨架 在细胞分化过程中的功能，细胞分化中的一个重要问题， 生物学研究