UW Biotechnology Center: Hybridoma and Transgenic Mouse Facilities

该提案要求资金用于增加现有的杂交瘤设施，并创建一个新的转基因小鼠设施。杂交瘤设施将包含设备和用品，并为生物研究所需的单克隆抗体的生产提供技术援助。主要项目包括：核糖体结构、RNA聚合酶sigma因子、肉毒杆菌毒素、雌激素受体、核小核糖核蛋白颗粒的研究。转基因小鼠设施将提供服务，并提供转基因小鼠的生产和遗传分析方面的专业知识。该设施的主要目标是生产用于研究人类遗传疾病的小鼠模型和研究生理过程的遗传控制或转基因小鼠模型，用于研究在改变食品生产动物的效率或产品组成方面重要的基因。建议使用Support。

This proposal requests funds to augment the existing Hybridoma Facility and to create a new Transgenic Mouse Facility. The Hybridoma Facility will contain equipment and supplies as well as provide the technical assistance for the production of monoclonal antibodies required for biological research. Major projects include: studies of the structure of ribosomes, RNA polymerase sigma factors, Botulism toxin, estrogen receptors, and small nuclear ribonucleoprotein particles. The Transgenic Mouse Facility will perform as a service and provide expertise in the production and genetic analysis of transgenic mice. The principal objectives of the facility are to produce mouse models for the study of human genetic disorders and to study the genetic control of physiological processes or transgenic mouse models for the study of genes that are important in altering the efficiency or product composition of food-producing animals. Support is recommended.