Ultrastructure, Photosynthetic Pigments, and Nutritional Characteristics of Oceanic Ultraplankton Clones in Culture

真核超浮游生物（1-6 + um）有时是最多的 光合浮游生物的重要组成部分，无论是在 生物量和初级生产力，特别是深海 叶绿素最大值（ca. 100米深）。 组成它的物种 由于两个原因，研究很少;除了他们的 体积小，它们脆弱性使得大多数保存不佳， 常规观察，直到最近， 使隔离成为文化非常困难。 我们 超浮游生物收集现在包含大约200个单藻克隆 或纯培养。 我们正在研究的合作伙伴（1） 光镜、扫描电镜和透射电镜观察形态和超微结构， （2）利用高效液相色谱法和 类胡萝卜素化学 结果将有助于将 毫无疑问会产生新的分类群， 新的分类标准，并可能重大修订的一些 更高的系统水平。 我们还研究了克隆体的营养 在文化中。 有些具有异养或噬菌体 能力的 微量元素要求-铁、锰、锌、硒- 将与已知的海洋和浅海的要求进行比较， 浮游植物物种的生长研究在规定的条件下。 这些研究以及其他机构正在进行的研究， 光合作用行为、其他生理 财产，放牧，等等，将使我们更好地了解 这一重要植物群的生态学，现在可供研究。

The eukaryotic ultraplankton (1-6 + um) is at times the most signigicant component of the photosynthetic plankton, both in terms of biomass and primary productivity, especially in the deep chlorophyll maximum (ca. 100 m depth). The species comprising it have been studied very little for two reasons; besides their small size, their fragility makes most preserve poorly for routine observation, and their sensitivity has until recently made isolation into culture exceedingly difficult. Our ultraplankton collection now contains some 200 clones in unialgal or axenic culture. With collaborators we are studying (1) morphology and ultrastructure by light microscopy, SEM and TEM, and (2) photosynthetic pigment suites by HPLC and techniques of carotenoid chemistry. The results will assist in placing the organisms taxonomically and undoubtedly will result in new taxa, new taxonomic criteria, and possibly major revision of some higher systematic levels. We also study nutrition of the clones in culture. Some have heterotrophic or phagotrophic capabilities. Requirements for trace elements - Fe, Mn, Zn, Se - will be compared to the requirements of known oceanic and neritic phytoplankton species by growth studies under defined conditions. These studies, together with those being carried out by other groups on photosynthetic behavior, other physiological properties, grazing, etc. will give us much better understanding of the ecology of this important flora, now available for study.