The Tobacco Ringspot Virus and its Associated Satellite in Plant Protoplasts Infected by Electroporation

一些RNA植物病毒与RNA相关，称为 小卫星RNA CA。360核苷酸残基 烟草环斑病毒卫星RNA 不是信使RNA，也没有广泛的核苷酸 与其支持病毒烟草环斑病毒的序列同源性 病毒（TobRV），如通过杂交分析检测的。 然而，STobRV RNA在TobRV感染的植物中复制 在那里它减少了TobRV的积累和症状的严重程度， 在TobRV外壳蛋白中被包裹。 多聚形式和 在感染组织中发现的环状STobRV RNA， 可能是复制中间体。 本研究的目的是 开发电穿孔程序来感染植物细胞 用TobRV和STobRV感染原生质体，并研究其作用机理 卫星RNA在原生质体中的复制。 这些研究 将允许阐明的相互作用和关系 在这个系统中基因组和卫星RNA之间， 使原生质体中的这些关系能够与 那些完整的植物。

Some RNA plant viruses become associated with RNAs termed "small satellite RNAs". The ca. 360 nucleotide residue satellite RNA of tobacco of tobacco ringspot virus (STobRV RNA) is not a messenger RNA, and it has no extensive nucleotide sequence homology with its supporting virus, tobacco ringspot virus (TobRV), as detected by hybridization analysis. Nevertheless, STobRV RNA replicates in TobRV infected plants where it reduces TobRV accumulation and symptom severity and becomes encapsidated in TobRV coat protein. Multimeric and circular forms of STobRV RNA found in infected tissues are likely replication intermediates. The goals of this study are to develop electroporation procedures to infect plant cell protoplasts with TobRV and STobRV and to study the mechanism of replication of the satellite RNA in protoplasts. These studies will allow elucidation of the interactions and relationships between the genomic and satellite RNAs in this system and will enable these relationships in protoplasts to be compared with those of intact plants.