Redox Proteins of the Membranes of Glyoxysomes

乙醛酸体膜的氧化还原蛋白

• 批准号: 8716740
• 负责人: Robert Donaldson
• 金额: $ 22.3万
• 依托单位: George Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8716740&HistoricalAwards=false
• 关键词: Redox; Proteins; Membranes; Glyoxysomes

A redox system is present in the membranes of glyoxsomes from the endosperm of germinating castor bean (Ricinus communis L.). This electron transport system is thought to consist of a transmembrane flavoprotein (NADH:ferrichyanide reductase) and cytochrome b5 in the cytoplasmic face of the membrane. It is hypothesized that the flavoprotein accepts electrons from NADH at the matrix face and transfers those electrons to cytochrome b5 or a cytosolic acceptor at the cytoplasmic face. The protein responsible for the NADH:ferricyanide reductase activity will be isolated from glyoxysomal membranes and characterized. Another redox protein, cytochrome b5, will also be isolated. The kinetic characteristics of the isolated proteins will be compared to the redox activities in the intact glyoxysomal membranes. Possible endogenus electron acceptors will be identified. Antibodies to the redox proteins will be generated and used together with inhibitors and non-penetrating probes to determine the orientations of the proteins in the membrane. The proposed work is important to understand the mechanism by which plants generate the energy and metabolites necessary to begin the germination process. Past work discovered a new energy shuttling mechanism within glyoxysomes. The proposal work will determine its stucture and specific operation within the cell compartment.//

氧化还原系统存在于乙二醛体的膜中 从发芽的蓖麻子（Ricinus communis L.）。 这种电子传输系统被认为是由一个 跨膜黄素蛋白（NADH：铁氰化物还原酶）和 细胞色素b5在细胞膜的细胞质面。 是 假设黄素蛋白接受来自NADH的电子， 基质面对并将这些电子转移到细胞色素b5， 位于胞质表面的胞质受体。 蛋白质 负责NADH：铁氰化物还原酶活性的蛋白质将是 从乙醛酸酶体膜分离并表征。 另一 还将分离氧化还原蛋白，细胞色素b5。 动力学 分离的蛋白质的特征将与 氧化还原活性在完整的乙醛酸酶体膜。 可能 将鉴定内生电子受体。 抗体 氧化还原蛋白将被产生并与 抑制剂和非穿透性探针，以确定 蛋白质在膜中的取向。 建议的工作是重要的了解机制 植物通过它产生能量和代谢物， 开始发芽过程。 过去的工作发现了一种新的能量 在乙醛酸酶体内的穿梭机制。 提案工作将 确定其结构和在细胞内的具体操作 compartment.