Glucocorticoid Receptor: Structure, Expression, and Signal Transduction

糖皮质激素受体：结构、表达和信号转导

• 批准号: 8716787
• 负责人: Keith Yamamoto
• 金额: $ 40.74万
• 依托单位: University of California-San Francisco
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-15 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8716787&HistoricalAwards=false
• 关键词: Glucocorticoid; Receptor; Structure; Expression; Signal

Glucocorticoid receptors transduce hormonal signalling information into specific alterations in gene transcription. We have cloned the rat glucocorticoid receptor coding sequences and characterized its functional domains. Here we propose: (1) to clone genomic receptor sequences for wild type and mutant receptor genes, characterizing the receptor promoter and its expression and regulation, and attempting to relate receptor gene organization to its evolution and function; (2) to pursue the mechanism of signal transduction, characterizing in particular the DNA binding and nuclear localization activities of the receptor, and selecting mutants in the overall process of transduction; and (3) to produce and purify preparative amounts of intact receptor and receptor fragments for biochemical and macromolecular structural analyses of the receptor, receptor- hormone and receptor-DNA complexes. These studies will broaden our understanding of the mechanisms of signal transduction and transcriptional regulation.

糖皮质激素受体调节激素信号 信息转化为基因转录中的特定改变。 我们克隆了大鼠糖皮质激素受体编码区 序列并表征其功能结构域。 这里我们 目的：（1）克隆野生型的基因组受体序列 型和突变型受体基因，表征受体 启动子及其表达和调控，并试图 将受体基因组织与其进化联系起来， 功能;（2）追求信号机制 转导，特别是表征DNA结合 和受体的核定位活性，以及 在整个转导过程中选择突变体;以及 (3)以生产和纯化制备量的完整的 用于生物化学和生物医学的受体和受体片段 受体的大分子结构分析，受体- 激素和受体-DNA复合物。 这些研究将扩大我们对 信号转导和转录机制 调控