Structural Requirements for Protein Membrane Assembly

蛋白质膜组装的结构要求

• 批准号: 8718578
• 负责人: Ross Dalbey
• 金额: $ 17.89万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8718578&HistoricalAwards=false
• 关键词: Structural; Requirements; Protein; Membrane; Assembly

This is a proposal to explore in detail leader peptidase, an integral transmembrane protein, to determine the exact requirements for membrane insertion and translocation of the c- terminal domain of this enzyme onto the outside of the Eescheria coli inner membrane. Site-directed mutagenesis and gene cloning techniques will be used to define and characterize the assembly elements within membrane proteins. The study will contribute to a better understanding of protein targeting and membrane assembly in cells and ultimately, might the help understand molecular basis of many diseases.

这是一个建议，探讨详细的领导肽酶， 完整的跨膜蛋白，以确定确切的 膜插入和易位的c- 这种酶的末端结构域的外部 大肠杆菌内膜。 定点诱变和 基因克隆技术将用于定义和表征 膜蛋白中的组装元件。 这项研究将 有助于更好地理解蛋白质靶向， 细胞中的膜组装，最终可能会有所帮助 了解许多疾病的分子基础。