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The Role of Acidic Residues and the Electrochemical Potential in Membrane Protein Assembly

酸性残基和电化学势在膜蛋白组装中的作用

基本信息

批准号：
9728344
负责人：
Ross Dalbey
金额：
$ 7.5万
依托单位：
Ohio State University Research Foundation -DO NOT USE
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1998
资助国家：
美国
起止时间：
1998-03-15 至 1998-11-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728344&HistoricalAwards=false
关键词：
Role Acidic Residues Electrochemical Potential

项目摘要

9728344 Dalbey Lay Abstract A fundamental process in cell biology is the way in which proteins are inserted into cellular membranes and achieve their distinctive and unique topology within the membrane. This project is based on studies of bacteria in this laboratory and the discovery that negatively charged residues within certain bacterial membrane proteins play an active role in membrane insertion. This insertion apparently requires only the energy of the electrochemical gradient across the membrane. The hypothesis is that the electrochemical gradient promotes insertion by the direct driving force of the membrane potential on the negatively charged amino acids in the specific regions of the membrane protein that will be translocated. This is in contrast to other mechanisms of insertion in other systems in which insertion of specific proteins requires a complex set of additional proteins that must recognize and interact with the translocated protein. The novel hypothesis will be tested by examining the insertion of a variety of bacterial proteins and hybrid protein constructs that are mutated at specific residues thought to play a role in translocation. The membrane pH and potential will be changed to assess the role of these factors on transport. This research on the mechanism of insertion into bacterial membranes will likely be fundamentally important in the understanding of similar processes in mitochondria of eukaryotic cells since these cell organelles are believed to be evolutionarily derived from symbiotic bacteria. ***

细胞生物学的一个基本过程是蛋白质插入细胞膜并在膜内实现其独特的拓扑结构。这个项目是基于这个实验室对细菌的研究，以及在某些细菌膜蛋白中带负电荷的残基在膜插入中起积极作用的发现。这种插入显然只需要跨膜的电化学梯度的能量。假设是电化学梯度通过膜电位的直接驱动作用于带负电荷的氨基酸在膜蛋白的特定区域将被易位，从而促进插入。这与其他系统中的其他插入机制形成对比，在其他系统中，特定蛋白质的插入需要一组复杂的附加蛋白质，这些蛋白质必须识别易位蛋白质并与之相互作用。这一新的假设将通过检测各种细菌蛋白质和杂交蛋白质结构的插入来验证，这些蛋白质在被认为在易位中起作用的特定残基上发生突变。将改变膜的pH值和电位，以评估这些因素在运输中的作用。这项关于细菌膜插入机制的研究可能对理解真核细胞线粒体的类似过程至关重要，因为这些细胞器被认为是从共生细菌进化而来的。＊＊＊