Phosphorus Metabolism in Methanogenic Bacteria

产甲烷细菌中的磷代谢

• 批准号: 8719737
• 负责人: David Fahrney
• 金额: $ 18.88万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-15 至 1991-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8719737&HistoricalAwards=false
• 关键词: Phosphorus; Metabolism; Methanogenic; Bacteria

The funded research focuses on the role of two major phosphorus compounds in Methanobacterium thermoautotrophicum and Methanothermus fervidus. These thermophilic methanogens have optimal growth temperatures of 65 and 84 C, respectively. The major focus is on the synthesis and function of cyclic 2,3- diphosphoglycerate (cyclic DPG), the first example of a cyclic pyrophosphate from either biological or nonbiological origins. Cyclic DPG levels range from 70 mM in M. thermoautotrophicum to 300 mM in M. fervidus, indicating a major role in thermal adaptation. Tracer experiments with radioactive phosphorus will provide important information on the turnover of cyclic DPG and indicate whether it might have a role in energy flow in mesophilic methanogens and a dual role in thermophilic methanogens. Recently this lab has discovered a large (36 mM) long-chain polyphosphate pool in M. thermoautotrophicum; this pool has a half-life under 10 minutes, indicating a major role in energy flow. The main goal is the identification and characterization of the enzymes in the cyclic DPG and polyphosphate pathways, with special attention to the possible role of the cyclic DPG enzymes as a thermal sensing system. Elucidation of these pathways will contribute significantly to our understanding of the biochemistry of methanogens and could have significant impact on the application of methanogens in biotechnology.*** //

该资助的研究重点是两种主要磷化合物在嗜热自养甲烷杆菌和产热甲烷嗜热菌中的作用。 这些嗜热产甲烷菌的最佳生长温度分别为 65 和 84 C。 主要重点是环状 2,3-二磷酸甘油酸酯（环状 DPG）的合成和功能，这是生物或非生物来源的环状焦磷酸盐的第一个例子。 循环 DPG 水平范围从 M.thermoautotropicum 中的 70 mM 到 M. fervidus 中的 300 mM，表明在热适应中发挥着重要作用。 放射性磷的示踪实验将提供有关环状 DPG 周转的重要信息，并表明它是否可能在中温产甲烷菌的能量流中发挥作用，并在嗜热产甲烷菌中发挥双重作用。 最近，本实验室在热自养分枝杆菌中发现了一个大型（36 mM）长链多磷酸盐池；该池的半衰期不到 10 分钟，表明在能量流中发挥着重要作用。 主要目标是对环状 DPG 和多磷酸途径中的酶进行鉴定和表征，特别关注环状 DPG 酶作为热传感系统的可能作用。 这些途径的阐明将极大地促进我们对产甲烷菌生物化学的理解，并可能对产甲烷菌在生物技术中的应用产生重大影响。*** //