Understanding syntrophic hydrocarbon metabolism under methanogenic conditions
了解产甲烷条件下的互养碳氢化合物代谢
基本信息
- 批准号:RGPIN-2015-05214
- 负责人:
- 金额:$ 2.77万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2019
- 资助国家:加拿大
- 起止时间:2019-01-01 至 2020-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Hydrocarbon spills into subsurface environments such as groundwater aquifers frequently lead to the development of methanogenic conditions. Many studies have shown that hydrocarbons can be biodegraded to methane by microbes, suggesting that we may be able to rely on such microbial activity for contaminated site clean up. To do so, we must have a better understanding of how this metabolism operates in order to determine the advantages and limitations of relying on methanogenic consortia for remediation. Syntrophy, meaning `feeding together', is a mode of microbial metabolism wherein different species must cooperate (for thermodynamic reasons) to carry out metabolic processes. Syntrophic microbial processes play key roles in large-scale beneficial industrial processes such as waste water treatment and make significant contributions to carbon cycling in anaerobic ecosystems. As such, syntrophy has mainly been studied using microbes that metabolize relatively simple carbon sources (such as fatty acids). As with fatty acids, methanogenic hydrocarbon metabolism must occur via syntrophic processes wherein cooperation among microbial species is needed to break down a given hydrocarbon to carbon dioxide and methane. However, little is known about the ecological principles of syntrophy in hydrocarbon-containing methanogenic ecosystems. Thus, the long-term objective of this research program is to elucidate the fundamental scientific principles underlying syntrophic hydrocarbon metabolism under methanogenic conditions that will be accomplished via two major short-term objectives. Objective 1 will be to improve upon and/or develop new strategies for cultivating syntrophic hydrocarbon-degrading methanogenic consortia in order to decrease lag times and improve rates of metabolism to enable more efficient study of syntrophic hydrocarbon metabolism. This objective will use a cultivation approach to test the use of various medium additives, solid supports, and growth conditions to improve rates of metabolism. Objective 2 will be to identify and quantify the key syntrophy genes and hydrocarbon-degrading genes that are expressed during methanogenic hydrocarbon metabolism using molecular biology approaches. This information is currently unknown. The proposed research will address major gaps in the fields of syntrophy and methanogenic hydrocarbon metabolism, leading to fundamental scientific discoveries and potential applications for contaminated site remediation. The outcome of the research will be of great benefit to Canada by helping to ensure clean environments (such as groundwater resources). Through the proposed program, many highly qualified personnel will be trained in diverse technical skills (ranging from analytical chemistry to genomics) and soft skills (leadership, communication) that will position them well for jobs in academia, industry, or government.
碳氢化合物溢出到地下环境,如地下水含水层,经常导致产甲烷条件的发展。 许多研究表明,微生物可以将碳氢化合物生物降解为甲烷,这表明我们可以依靠这种微生物活性来清理污染场地。 要做到这一点,我们必须更好地了解这种代谢是如何运作的,以确定依靠产甲烷财团进行修复的优势和局限性。共养,意思是“共同进食”,是微生物代谢的一种模式,其中不同物种必须合作(出于热力学原因)来进行代谢过程。 互养微生物过程在废水处理等大规模有益的工业过程中发挥着关键作用,并对厌氧生态系统中的碳循环做出了重大贡献。 因此,主要使用代谢相对简单的碳源(如脂肪酸)的微生物来研究合成营养。 与脂肪酸一样,产甲烷烃代谢必须通过互养过程发生,其中需要微生物物种之间的合作将给定的烃分解为二氧化碳和甲烷。 然而,对含烃产甲烷生态系统中的互养生态学原理知之甚少。 因此,本研究计划的长期目标是阐明产甲烷条件下互养烃代谢的基本科学原理,这将通过两个主要的短期目标来实现。 目标1将是改进和/或开发用于培养互养烃降解产甲烷聚生体的新策略,以减少滞后时间并提高代谢速率,从而能够更有效地研究互养烃代谢。该目标将使用培养方法来测试各种培养基添加剂、固体支持物和生长条件的使用,以提高代谢速率。 目标二是利用分子生物学方法鉴定和定量分析产甲烷烃代谢过程中表达的关键合成营养基因和烃降解基因。这一信息目前尚不清楚。拟议的研究将填补合成营养和产甲烷碳氢化合物代谢领域的主要空白,导致基础科学发现和污染场地补救的潜在应用,研究成果将有助于确保清洁环境(如地下水资源),对加拿大大有裨益。通过拟议的计划,许多高素质的人才将接受各种技术技能(从分析化学到基因组学)和软技能(领导力,沟通)的培训,这些技能将使他们能够在学术界,工业界或政府工作。
项目成果
期刊论文数量(0)
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会议论文数量(0)
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{{ truncateString('Gieg, Lisa', 18)}}的其他基金
Team Play: Determining Syntrophic Interactions in Hydrocarbon Biodegradation Processes
团队合作:确定碳氢化合物生物降解过程中的互养相互作用
- 批准号:
RGPIN-2020-07029 - 财政年份:2022
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Team Play: Determining Syntrophic Interactions in Hydrocarbon Biodegradation Processes
团队合作:确定碳氢化合物生物降解过程中的互养相互作用
- 批准号:
RGPIN-2020-07029 - 财政年份:2021
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Sustainable crude oil recovery: Advancing technology in microbial souring control and microbial enhanced oil recovery
可持续原油采收:微生物酸化控制和微生物采收率先进技术
- 批准号:
531772-2018 - 财政年份:2021
- 资助金额:
$ 2.77万 - 项目类别:
Collaborative Research and Development Grants
Team Play: Determining Syntrophic Interactions in Hydrocarbon Biodegradation Processes
团队合作:确定碳氢化合物生物降解过程中的互养相互作用
- 批准号:
RGPIN-2020-07029 - 财政年份:2020
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Sustainable crude oil recovery: Advancing technology in microbial souring control and microbial enhanced oil recovery
可持续原油采收:微生物酸化控制和微生物采收率先进技术
- 批准号:
531772-2018 - 财政年份:2020
- 资助金额:
$ 2.77万 - 项目类别:
Collaborative Research and Development Grants
Sustainable crude oil recovery: Advancing technology in microbial souring control and microbial enhanced oil recovery
可持续原油采收:微生物酸化控制和微生物采收率先进技术
- 批准号:
531772-2018 - 财政年份:2019
- 资助金额:
$ 2.77万 - 项目类别:
Collaborative Research and Development Grants
Understanding syntrophic hydrocarbon metabolism under methanogenic conditions
了解产甲烷条件下的互养碳氢化合物代谢
- 批准号:
RGPIN-2015-05214 - 财政年份:2018
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Understanding syntrophic hydrocarbon metabolism under methanogenic conditions
了解产甲烷条件下的互养碳氢化合物代谢
- 批准号:
RGPIN-2015-05214 - 财政年份:2017
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Understanding syntrophic hydrocarbon metabolism under methanogenic conditions
了解产甲烷条件下的互养碳氢化合物代谢
- 批准号:
RGPIN-2015-05214 - 财政年份:2016
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Prospects for anaerobic benzene bioremediation in contaminated aquifers
受污染含水层厌氧苯生物修复的前景
- 批准号:
507645-2016 - 财政年份:2016
- 资助金额:
$ 2.77万 - 项目类别:
Engage Grants Program
相似海外基金
Team Play: Determining Syntrophic Interactions in Hydrocarbon Biodegradation Processes
团队合作:确定碳氢化合物生物降解过程中的互养相互作用
- 批准号:
RGPIN-2020-07029 - 财政年份:2022
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Team Play: Determining Syntrophic Interactions in Hydrocarbon Biodegradation Processes
团队合作:确定碳氢化合物生物降解过程中的互养相互作用
- 批准号:
RGPIN-2020-07029 - 财政年份:2021
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Team Play: Determining Syntrophic Interactions in Hydrocarbon Biodegradation Processes
团队合作:确定碳氢化合物生物降解过程中的互养相互作用
- 批准号:
RGPIN-2020-07029 - 财政年份:2020
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Understanding syntrophic hydrocarbon metabolism under methanogenic conditions
了解产甲烷条件下的互养碳氢化合物代谢
- 批准号:
RGPIN-2015-05214 - 财政年份:2018
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Understanding syntrophic hydrocarbon metabolism under methanogenic conditions
了解产甲烷条件下的互养碳氢化合物代谢
- 批准号:
RGPIN-2015-05214 - 财政年份:2017
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Understanding syntrophic hydrocarbon metabolism under methanogenic conditions
了解产甲烷条件下的互养碳氢化合物代谢
- 批准号:
RGPIN-2015-05214 - 财政年份:2016
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Understanding syntrophic hydrocarbon metabolism under methanogenic conditions
了解产甲烷条件下的互养碳氢化合物代谢
- 批准号:
RGPIN-2015-05214 - 财政年份:2015
- 资助金额:
$ 2.77万 - 项目类别:
Discovery Grants Program - Individual
Characterization of syntrophic microorganisms and metabolic pathways involved in methanogenic hydrocarbon biodegradation
产甲烷碳氢化合物生物降解涉及的互养微生物和代谢途径的表征
- 批准号:
410195-2011 - 财政年份:2013
- 资助金额:
$ 2.77万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Doctoral
Characterization of syntrophic microorganisms and metabolic pathways involved in methanogenic hydrocarbon biodegradation
产甲烷碳氢化合物生物降解涉及的互养微生物和代谢途径的表征
- 批准号:
410195-2011 - 财政年份:2012
- 资助金额:
$ 2.77万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Doctoral
Characterization of syntrophic microorganisms and metabolic pathways involved in methanogenic hydrocarbon biodegradation
产甲烷碳氢化合物生物降解涉及的互养微生物和代谢途径的表征
- 批准号:
410195-2011 - 财政年份:2011
- 资助金额:
$ 2.77万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Doctoral