U.S.-Switzerland Cooperative Research: Characterization of Host-Selective Toxins from Plant Pathogenic Fungi

该奖项支持弗拉基米尔·马科博士的几次研究访问， 康奈尔大学博伊斯汤普森研究所，瑞士联邦 苏黎世理工学院，以便与 Duilio Arigoni博士在化学和生物学的研究， 植物病原真菌的寄主选择性毒素。 协作 Arigoni博士，世界知名的天然产物化学家，将 加强Macko博士长期在国内对 这些毒素的作用是针对获得更好的 在分子水平上理解宿主-病原体相互作用。 他们提出的研究的具体实验目标是： 1)分离和鉴定来自于宿主选择性毒素的 植物病原真菌Periconia circinata（PC）; 2）建立 PC毒素的结构-活性关系;以及3） 制备一种放射性标记的PC毒素， 在其作用位点（受体）在宿主中的未来研究中 厂 大部分工作将在美国进行。 实验室 将用各种方法进行光谱分析 在两个实验室里，阿里戈尼博士几乎是独一无二的 解释生物活性化合物光谱的专业知识将是 非常有价值 Arigoni博士将进行平行研究， 活性化合物的化学和生物合成， 他们的前身，伴随着样品，思想和 与Macko博士的信息 尽管现代定量生物学的贡献， 生理生化分析进展 宿主-病原体相互作用，我们缺乏对 毒素的特异性和耐药性的分子机制， 易感性 分子基础的阐明 宿主-病原体相互作用的特异性是最重要的 现代植物病理学的重要目标，因为它 将有助于识别和操纵疾病反应基因， 及其产品。 人们对生物活性物质的兴趣也越来越大。 天然产物，因为它们在 农业和生物技术。

This award supports several research visits by Dr. Vladimir Macko, Boyce Thompson Institute of Cornell University, to the Swiss Federal Institute of Technology in Zurich in order to collaborate with Dr. Duilio Arigoni in studies of the chemistry and biology of host-selective toxins from plant pathogenic fungi. Collaboration with Dr. Arigoni, a world reknowned natural products chemist, will enhance Dr. Macko's long-term domestic research on the mechanism of action of these toxins which is directed at gaining a better understanding of host-pathogen interactions at the molecular level. The specific experimental objectives of their proposed research are: 1) to isolate and characterize the host-selective toxins from the plant pathogenic fungus Periconia circinata (PC); 2) to establish the structure-activity relationship for the PC toxins: and 3) to prepare a radioactively labeled PC toxin that could serve as a probe in future studies of its site of action (receptor) in the host plant. Most of this work will be carried out in the U.S. laboratory. Spectral analysis by various methods will be carried out in both laboratories, and Dr. Arigoni's virtually unique expertise in interpreting spectra of the bioactive compounds will be extremely valuable. Dr. Arigoni will carry out parallel research on the chemical and biological synthesis of the active compounds and their precursors, with concomitant exchange of samples, ideas and information with Dr. Macko. Despite the contributions of modern, quantitative biology to advances in the analysis of the physiology and biochemistry of host-pathogen interactions, we lack a basic understanding of specificity in toxins and the molecular mechanisms of resistance and susceptibility. The elucidation of the molecular basis of specificity in host-pathogen interactions is one of the most important objectives in contemporary plant pathology, because it will help in identifying and manipulating the disease reaction genes and their products. There is also increasing interest in bioactive natural products because of their potential applications in agriculture and biotechnology.