U.S.-Federal Republic of Germany Cooperative Research on Genetics of Mobile Elements in Maize

该奖项支持彼得博士的三次短期研究访问 彼得森的爱荷华州州立大学的马克斯普朗克研究所 德国科隆的植物育种研究，以提高他的国内 与海因茨塞德勒博士和其他人合作进行研究。 的 他们共同工作的目的是更好地了解 玉米转座和基因表达的调控。 他们的 研究计划包括努力1）进一步调查 新出现的不稳定等位基因与目前已知的 突变系统; 2）通过分离突变体来分析En系统 控制En元件的若干特定功能; 3)在几个基因座上进行可变等位基因的遗传分析， 为了分离突变体衍生物用于另外的分子 研究; 4）分析玉米种群的分布， 突变元件;和5）在克隆的基因座处捕获主元件， 它们在标记基因中的用途。 一个协调的遗传（爱荷华州）和 分子（德国）的方法是必要的大部分这项工作。 彼得森博士有所有的设施和基因信息， 操纵遗传物质的技术 Saedler博士的团队 分子生物学技术发达， 进行分子水平的分析。 玉米中移动的元素的研究已经取得了相当大的进展 在过去的几年里，随着分离和克隆几个 元件和随后的一些基因的克隆。 这 进展归功于分子的共同努力， 生物学家和遗传学家，包括这些合作者。 早期 工作阐明了元素的一般结构， 组成部分，以及它们的遗传效应。 这项工作产生了 开发模型来解释这些元素在 诱导基因组和单个基因的变化。 的 拟议的合作将进一步开展这项工作， 最后，我们进行了标记理想基因的实验， 对农业很重要。

This award supports three short term research visits by Dr. Peter Peterson of Iowa State University to the Max-Planck Institute for Plant Breeding Research in Cologne, FRG, to enhance his domestic research by collaboration with Dr. Heinz Saedler and others. The objective of their joint work is to gain a better understanding of the regulation of transposition and gene expression in maize. Their research plans include efforts to 1) investigate further the relationship of newly arisen unstable alleles to currently known mutable systems; 2) analyze the En system by isolating mutants controlling the several specific functions of the En element; 3) pursue the genetic analysis of mutable alleles at several loci in order to isolate mutant derivatives for additional molecular studies; 4) analyze populations of maize for the distribution of mutable elements; and 5) trap master elements at cloned loci for their use in tagging genes. A coordinated genetic (Iowa) and molecular (Germany) approach is necessary for most of this work. Dr. Peterson has all the facilities and genetic information and techniques to manipulate the genetic material. Dr. Saedler's group has the molecular biology technology well developed and has personnel to carry out the molecular level analyses. Studies of mobile elements in maize have made considerable progress in the past few years, with the isolation and cloning of several elements and the subsequent cloning of a number of genes. This progress is attributable to the combined efforts of molecular biologists and geneticists, including these collaborators. Early work has elucidated the general structure of the elements, their component parts, and their genetic effects. This work has generated the development of models to explain the role of these elements in inducing changes in the genome as well as in individual genes. The proposed collaboration will carry this work further, leading eventually to experiments on tagging desirable genes that are important to agriculture.