Immunology--A New Capstone Course for Undergraduate Biology Majors.

免疫学——本科生物学专业的新顶点课程。

• 批准号: 8750098
• 负责人: David Oetinger
• 金额: $ 1.51万
• 依托单位: Kentucky Wesleyan College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-06-15 至 1989-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8750098&HistoricalAwards=false
• 关键词: Immunology; New; Capstone; Course; Undergraduate

A new capstone course required of biology majors is being created. Immunology is replacing the course which has served a capstone function for the past 22 years, Plant Physiology. Immunofluorescence electrophoretic procedures, and cell culture--hybridoma production are becoming part of a revised Immunology laboratory curriculum. This revised curriculum is seeking to ensure that biology majors are knowledgeable and well-trained in the areas of experimental design; discipline in experimental work; care of living organisms; precision in manipulative work; use of instrumentation; data collection; data analysis; and report writing. Microscopes, protein electrophresis and immunoductrophoresis equipment are being used by the students. Specifically, the immunology laboratory includes three new project areas in which students generate and test hypotheses: humoral immunity (producing antisera to soluble antigens, protein determinations, and electrophoretic/immunoelectrophoretic/- immunodiffusion demonstrations of antibody specificity and titer), immunolabelling (producing antisera to bacterial antigens, purifying and labelling antibodies and subsequent demonstration of their specificity with immunofluorescence), and cellular immunity" (demonstrating populations of T- and B-lymphocytes, culture of mouse myeloma cells, fusion of myeloma cells with stimulated mouse splenic lymphocytes, and attemps to identify monoclonal antibody- producing cells).

生物学专业所需的一门新的顶点课程正在创建中。 免疫学正在取代起着顶点作用的课程 在过去的22年里，植物生理学。 免疫荧光 电泳程序和细胞培养-杂交瘤生产是 成为修订后的免疫学实验室课程的一部分。 这 修订后的课程旨在确保生物学专业的学生 在实验设计领域知识渊博，训练有素; 实验工作的纪律;生物体的护理;精确度 操作工作;仪器的使用;数据收集;数据 分析;撰写报告。 显微镜、蛋白质电泳和 学生们正在使用免疫导管电泳设备。 具体来说，免疫学实验室包括三个新的项目领域 学生提出并检验假设：体液免疫 （生产可溶性抗原的抗血清，蛋白质测定， 电泳/免疫电泳/免疫扩散演示 抗体特异性和滴度），免疫标记（产生抗血清 细菌抗原，纯化和标记抗体， 用免疫荧光证明其特异性），以及 细胞免疫”（证明T淋巴细胞和B淋巴细胞的群体， 小鼠骨髓瘤细胞的培养，骨髓瘤细胞与刺激的 小鼠脾淋巴细胞，并用ELISA法鉴定单克隆抗体- 生产细胞）。