Instrumentation for a Newly Revised Undergraduate Biochemistry Laboratory Course

新修订的本科生物化学实验室课程的仪器

• 批准号: 8750821
• 负责人: Jeffrey Cohlberg
• 金额: --
• 依托单位: California State University-Long Beach
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-06-15 至 1989-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8750821&HistoricalAwards=false
• 关键词: Instrumentation; Newly; Revised; Undergraduate; Biochemistry

A new curriculum for a one-semester biochemistry laboratory course has been designed and is being implemented by the Chemistry Department at California State University at Long Beach. The course is the capstone of a newly approved degree program leading to a Bachelor of Science in Biochemistry. The course emphasizes the acquisition of both experience with modern biochemical research techniques and instrumentation and the acquisition of problem-solving skills used in a research setting. The following equipment has been recently added to the department's instrumental holdings, specifically for use in this regard: a high speed centrifuge, three ultraviolet-visible scanning spectrophotometers, a high pressure liquid chromatography gradient system with variable wavelength detector, and three low pressure liquid chromatography systems. In addition to experiments on protein determination, enzyme kinetics, and gel electrophoresis, the isolation and characterization of hemoglobin, DNA, and messenger RNA, from the brine shrimp Artemia Salina, is carried out, together with experiments on the transformation of bacteria with recombinant plasmids and the isolation and characterization of the plasmids.

加州州立大学长滩分校化学系为一学期的生物化学实验课程设计并实施了一套新课程。该课程是新批准的生物化学学士学位课程的顶点课程。本课程强调获得现代生化研究技术和仪器的经验，并获得在研究环境中使用的解决问题的技能。最近，该部门专门用于这方面的仪器设备增加了以下设备：一台高速离心机，三台紫外可见扫描分光光度计，一台带可变波长检测器的高压液相色谱梯度系统和三台低压液相色谱系统。除了蛋白质测定、酶动力学、凝胶电泳等实验外，还对盐碱对虾（Artemia Salina）的血红蛋白、DNA和信使RNA进行了分离和表征，并对重组质粒转化细菌进行了实验，对质粒进行了分离和表征。