RUI: Assembly and Interactions of Intermediate Filaments

RUI：中间丝的组装和相互作用

• 批准号: 8904460
• 负责人: Jeffrey Cohlberg
• 金额: --
• 依托单位: California State University-Long Beach Foundation
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-07-06
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8904460&HistoricalAwards=false
• 关键词: RUI; Assembly; Interactions; Intermediate; Filaments

The structure, function, and cellular interactions of intermediate filaments (IFs) are being investigated. The project focusses on two specific areas: neurofilament assembly, which involves three different neurofilament proteins, and IF interactions with mitochondria. IF assembly will be addressed by examining soluble assembly intermediates in vitro, using electrophoresis, analytical ultracentrifugation, and electron microscopy. Interactions with mitochondria will assessed in vitro using purified IFs and mitochondria. Intermediate filaments are arguably the most complex, and least understood, of the cytoskeletal elements of living cells. These experiments will pursue important aspects of the biochemistry of IFs, and will shed light on a plausible, though admittedly highly speculative, hypothesis concerning biological function.

人们正在研究中间纤维的结构、功能和细胞间的相互作用。该项目专注于两个特定领域：神经丝组装，涉及三种不同的神经丝蛋白，以及是否与线粒体相互作用。IF组装将通过检测体外可溶的组装中间产物、使用电泳法、分析性超速离心法和电子显微镜来解决。与线粒体的相互作用将使用纯化的IFS和线粒体在体外进行评估。在活细胞的细胞骨架元素中，中间丝可以说是最复杂的，也是最不被了解的。这些实验将探索生物系统生物化学的重要方面，并将阐明关于生物功能的一个看似合理的、尽管具有高度推测性的假说。