Polystyrene Microcarriers with Novel Surface Chemistry

学术和工业生物技术研究人员不断 寻找更好的材料来培养细胞。 拟议 研究将开发表面， 1. 促进锚定依赖的附着和生长 细胞 (e.g.上皮细胞），从而改善 这种效率 微载体培养 2. 允许非锚定依赖性细胞（即HeLa、CHO） 增长 载体结合以促进这些细胞的分离 和细胞培养产品。 私家侦探们用射频非平衡等离子体 化学修饰组织培养物表面的反应 板，并发现他们的修改造成显着 改善单层培养中多种细胞的生长。 的 专业人员被提议测试他们可以执行 在微载体珠上进行类似的修饰并实现相同的目的 这些珠粒上细胞生长的改善。 这将是第一次 朝着开发各种表面改性组织迈出了一步 培养基质，其将支持细胞生长，否则 不可能在体外生长，这将提高 大规模锚定依赖性细胞培养的效率。

Academic and industrial biotechnology researchers are continually seeking better materials on which to culture cells. The proposed research will develop surfaces which will; 1. Improve the attachment and growth of anchorage dependent cells (e.g. epithelial cells), thus improving the efficiency of such microcarrier cultures. 2. Allow non-anchorage dependent cells (i.e. HeLa, CHO) to grow carrier-bound to facilitate separation of these cells and cell culture products. The PIs have used radio-frequency, non-equilibrium plasma reactions to chemically modify the surface of tissue culture plates, and found that their modifications cause significantly improved growth of a variety of cells in monolayer culture. The PIs proposed to test the hypothesis that they can perform an analogous modification on microcarrier beads and achieve the same improvement in cell growth on these beads. This would be a first step toward development of a variety of surface modified tissue culture substrata which would support growth of cells otherwise impossible to grow in vitro, and which would improve the efficiency of large scale anchorage dependent cell culture.