An Engineering Approach to Microcarrier Surface Design

该项目的长期目标是开发具有新型表面的聚合物微载体，以支持细胞的生长，否则在体外很难或不可能生长。这些支架将在小规模的实验室细胞培养中有用，并且很容易适应大规模、高效的细胞和其他组织培养产品的生产。拟议的研究将允许改变微载体表面，独立于其体积性质，而无需后处理。这将通过改进众所周知的悬浮聚合技术来生产具有所需表面化学功能的微载体珠来实现。此外，支架的多功能性及其制造将能够为各种细胞创造最佳生长表面。

The long range goal of this project is to develop polymer based microcarriers with novel surfaces which will support the growth of cells otherwise difficult or impossible to grow in vitro. These supports will be useful in small scale laboratory cell culture and will be easily adpatable to the large scale, highly efficient manufacture of cells and other tissue culture products. The proposed research will allow alteration of the microcarrier surface, independent of its bulk properties, without the necessity of post-treatment. This will be accomplished by modifying well- known suspension polymerization techniques to produce microcarrier beads with the desired surface chemical functionalities. In addition, the versatility of the supports and their manufacture will enable the creation of optimal growth surfaces for a wide variety of cells.