An Engineering Approach to Microcarrier Surface Design

该项目的长期目标是开发基于聚合物的 具有新颖表面的微载体将支持 细胞难以或不可能在体外生长。 这些 支持物在小规模实验室细胞培养中是有用的， 将易于适应大规模、高效率 生产细胞和其他组织培养产品。 拟议的研究将允许改变微载体 表面，独立于其整体性质，而不需要 后处理。 这将通过修改来实现-- 制备微载体已知悬浮聚合技术 具有所需表面化学功能的珠粒。 在 此外，支撑件及其制造的多功能性 将能够创造最佳的生长表面， 各种细胞。

The long range goal of this project is to develop polymer based microcarriers with novel surfaces which will support the growth of cells otherwise difficult or impossible to grow in vitro. These supports will be useful in small scale laboratory cell culture and will be easily adpatable to the large scale, highly efficient manufacture of cells and other tissue culture products. The proposed research will allow alteration of the microcarrier surface, independent of its bulk properties, without the necessity of post-treatment. This will be accomplished by modifying well- known suspension polymerization techniques to produce microcarrier beads with the desired surface chemical functionalities. In addition, the versatility of the supports and their manufacture will enable the creation of optimal growth surfaces for a wide variety of cells.