An Improved Method of Microbial Cell Disruption

一种改进的微生物细胞破碎方法

• 批准号: 8760517
• 负责人: Trevor Castor
• 金额: $ 5万
• 依托单位: Aphios Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1988-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8760517&HistoricalAwards=false
• 关键词: Improved; Method; Microbial; Cell; Disruption

Narrative: Disruption of the cell envelope is one of the first steps in the recovery of materials contained within microbial cells. Conventional techniques typically involve high shear, heat generation, and multiple stage processing, all factors which can lead to degradation of the desired product. Furthermore, these techniques often lead to the creation of small cell wall fragments which adds to the burden of downstream purification. The present proposal suggests a method of cell disruption which may avoid these difficulties, improve product yields, and prove more economical as well. The method, a modified form of explosive decompression, is based on the large specific volume changes which may be rapidly effected upon depressurization of a supercritical fluid or low-boiling liquid. Any process requiring microbial cell disruption may benefit significantly from the proposed explosive disruption method.

叙述：破坏细胞包膜是微生物细胞内物质回收的第一步。传统技术通常涉及高剪切、产生热量和多阶段加工，所有这些因素都可能导致所需产品的降解。此外，这些技术经常导致产生小的细胞壁碎片，这增加了下游净化的负担。目前的建议提出了一种细胞破坏方法，可以避免这些困难，提高产品产量，并证明更经济。该方法是炸药减压的一种改进形式，其基础是在超临界流体或低沸点液体降压时可能迅速产生的较大比容变化。任何需要破坏微生物细胞的过程都可以从所提出的爆炸破坏方法中显著受益。