An Improved Method of Microbial Cell Disruption

叙述：细胞包膜的破坏是第一个 回收微生物中所含物质的步骤 细胞 常规技术通常涉及高剪切， 发热和多阶段处理， 可导致所需产物的降解。 此外，委员会认为， 这些技术通常导致产生小细胞壁 这增加了下游纯化的负担。 本发明提出了一种细胞破碎方法， 可以避免这些困难，提高产品产量，并证明 也更经济。 该方法是一种改进形式， 爆炸减压，是基于大的比容 在减压时可能迅速发生的变化 超临界流体或低沸点液体。 任何需要 微生物细胞破碎可显著受益于 提出了爆炸破坏法。

Narrative: Disruption of the cell envelope is one of the first steps in the recovery of materials contained within microbial cells. Conventional techniques typically involve high shear, heat generation, and multiple stage processing, all factors which can lead to degradation of the desired product. Furthermore, these techniques often lead to the creation of small cell wall fragments which adds to the burden of downstream purification. The present proposal suggests a method of cell disruption which may avoid these difficulties, improve product yields, and prove more economical as well. The method, a modified form of explosive decompression, is based on the large specific volume changes which may be rapidly effected upon depressurization of a supercritical fluid or low-boiling liquid. Any process requiring microbial cell disruption may benefit significantly from the proposed explosive disruption method.