RUI: The Influence of the Microenvironment on the Differentiation of Primary Mesenchyme Cells in the Sea Urchin Embryo

RUI：微环境对海胆胚胎原代间充质细胞分化的影响

• 批准号: 8801920
• 负责人: Stephen Benson
• 金额: $ 3.4万
• 依托单位: California State University, East Bay Foundation, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1990-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8801920&HistoricalAwards=false
• 关键词: RUI; Influence; Microenvironment; Differentiation; Primary

The objectives of this project are: 1) To examine the role of cell-cell interactions on the differentiation of micromeres of the sea urchin embryo, 2) To examine the effects of modifications of the extracellular matrix (ECM) environment of the embryo on the differentiation of the primary mesenchyme, 3) To characterize the ECM components synthesized by micromeres and primary mesenchyme in vivo and in vitro. Differentiation of these cells will be monitored by a) morphological appearance of spicules, b) quantitation of transcription of a spicule matrix gene, c) quantitation of accumulation of a spicule matrix protein mRNA, d) quantitation of synthesis of spicule matrix protein. %%% The sea urchin serves as an excellent model system for studying the early events in embryo development. Here Dr. Benson will look at the factors involved in the differentiation of the embryonic skeleton. He is at an undergraduate institution and the students will play a major part in this research as part of their training.

本项目的目标是：1）研究 细胞-细胞相互作用对小孢子分化的影响 海胆胚胎，2）检查修改的影响 胚胎的细胞外基质（ECM）环境 初级间充质的分化，3）表征 由微粒和初级合成的ECM组分 间充质在体内和体外。 这些细胞分化 将通过a）骨针的形态学外观，B） 骨针基质基因转录的定量，c） 骨针基质蛋白mRNA积累的定量，d） 骨针基质蛋白合成的定量。 %%% 海胆是一个很好的模型系统， 胚胎发育的早期事件。 在这里，本森博士将 看看参与差异化的因素， 胚胎骨骼他在一所本科院校， 学生将在这项研究中发挥重要作用，作为他们的一部分， 训练