Extracellular Matrix Induction of Primary Mesenchyme Differention

初级间充质分化的细胞外基质诱导

• 批准号: 9105913
• 负责人: Stephen Benson
• 金额: $ 11.55万
• 依托单位: California State University, East Bay Foundation, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-01-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105913&HistoricalAwards=false
• 关键词: Extracellular; Matrix; Induction; Primary; Mesenchyme

Sea urchin micromeres arise at the vegetal pole of the embryo by unequal cleavage in the vegetal tier of four cells. Primary mesenchyme, the cleavage products of the micromeres, migrate into the blastocoel of the embryo where they interact with the extracellular matrix (ECM) and differentiate to produce the embryonic skeleton or spicule. Evidence from in vitro and in vivo studies suggests that the micromeres require a permissive environment or signal to initiate differentiation. An intact embryonic ECM is a major component of the permissive environment. The PI has recently shown that micromeres will differentiate in vitro if cultured on a reconstituted basal lamina (matrigel). Isolated micromeres cultured in sea water alone will not differentiate. What is the molecule in the matrigel which initiates differentiation and how is it related to the endogenous signalling molecule in the embryo. The PI proposes to: (1) identify and purify the spicule inducing activity (SIA) from the matrigel, (2) characterize the general mechanism of action of SIA, and (3) identify and initiate characterization of the homologous SIA activity from the sea urchin embryo.

海胆微粒体出现在胚胎的植物极， 在四个细胞的植物层中不相等的分裂。 初级 间充质，分裂产物的微粒，迁移到 胚胎的囊胚腔，在那里它们与 细胞外基质（ECM）和分化产生的 胚胎骨骼或骨针。 来自体外和体内的证据 体内研究表明，微粒需要一个允许的 环境或信号启动分化。 完整 胚胎ECM是容许环境的主要成分。 PI最近表明，微粒细胞将分化， 如果在重构的基底层（基质胶）上培养，则为体外。 单独在海水中培养的分离的微粒不会 区分。 基质胶中的分子是什么 启动分化，以及它与内源性 胚胎中的信号分子。 PI建议：（1）鉴定和纯化骨针 诱导活性（SIA），（2）表征基质胶的 SIA的一般作用机制，以及（3）识别和启动 来自海洋的同源SIA活性的表征 海胆胚胎