RUI: Mechanisms of CO2 and Chloride Ion Translocation Across Isolated Perfused Crab Gills

RUI：二氧化碳和氯离子在离体灌注蟹鳃中易位的机制

• 批准号: 8802291
• 负责人: Louis Burnett
• 金额: $ 7.94万
• 依托单位: University of San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-10-15 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8802291&HistoricalAwards=false
• 关键词: RUI; Mechanisms; CO2; Chloride; Ion

The translocation of CO2 and chloride ions through gills will be studied using isolated perfused crab gills. CO2 flux across the gill is a process which can be complex due to several factors: 1) carbonic anhydrase, which catalyzes the hydration and dehydration reactions of CO2, is located on the cell surface and inside the cell; 2) CO2 is generated endogenously by active metabolic processes; and 3) CO2 hydrated to bicarbonate plays a role in chloride uptake via C1-/HCO3-exchange. The quantitative importance and contribution of the above processes to the excretion of CO2 out of the animal and the uptake of chloride during low salinity stress is poorly understood. This proposal outlines experiments testing hypotheses which will elucidate the dual (CO2 and C1-) and related translocation processes. This study, which has general relevance to ion uptake processes in any metabolically active epithelium, is designed to determine the existence and location of C1-/HCO3-exchanger, determine the amount and the fate of the CO2 produced by the gill, measure the quantitative contribution of endogenously produced CO2 to C1- /HCO3-exchange, and determine the quantitative function of cell surface and intracellular carbonic anhydrase in CO2 excretion and chloride uptake. The results have importance to our understanding of the interplay between acid balance, respiration and ion regulation of aquatic animals in normal and polluted waters.

CO2和氯离子通过鳃的易位将用分离的灌注蟹鳃进行研究。由于几个因素的影响，二氧化碳在鳃中的流动是一个复杂的过程：1)碳酸酐酶位于细胞表面和细胞内部，它催化二氧化碳的水合和脱水反应；2) CO2是通过主动代谢过程内源性产生的；3) CO2水合成碳酸氢盐，通过C1-/ hco3交换作用于氯化物的吸收。在低盐度胁迫下，上述过程对动物排出二氧化碳和吸收氯化物的数量重要性和贡献尚不清楚。本提案概述了实验测试的假设，这将阐明双重（CO2和C1-）和相关的易位过程。本研究与任何代谢活跃的上皮细胞的离子摄取过程普遍相关，旨在确定C1-/ hco3交换剂的存在和位置，确定鳃产生的CO2的数量和归宿，测量内源性CO2对C1-/ hco3交换的定量贡献。并确定细胞表面和细胞内碳酸酐酶在CO2排泄和氯化物摄取中的定量功能。研究结果对了解正常和污染水体中水生动物的酸平衡、呼吸和离子调节之间的相互作用具有重要意义。