RUI: Mechanisms of CO2 and Chloride Ion Translocation Across Isolated Perfused Crab Gills

二氧化碳和氯离子通过鳃的转移将是 使用离体灌注的蟹鳃进行研究。 CO2通量 鳃是一个复杂的过程，这可能是由于几个因素： 1)碳酸酐酶，它催化水合作用， CO2的脱水反应，位于细胞表面， 2）CO2是由活性碳内源性产生的 代谢过程;和3）CO2水合碳酸氢盐发挥作用， 通过C1-/HCO 3-交换在氯化物吸收中的作用。 定量 的重要性和贡献， 排出动物体内的CO2和吸收氯化物 在低盐胁迫下的作用机制知之甚少。 这项建议 概述了测试假设的实验， 双重（CO2和C1-）和相关的易位过程。 这 研究，这具有一般相关的离子吸收过程中的任何 代谢活性上皮，旨在确定 C1-/HCO 3-交换器的存在和位置，确定 量和命运的二氧化碳产生的鳃，测量 内源产生的CO2对C1- /HCO 3-交换，并测定细胞的定量功能 表面和细胞内碳酸酐酶在CO2排泄中的作用， 氯化物吸收 这些结果对我们的 了解酸平衡、呼吸作用 水生动物在正常和污染环境中的离子调节作用 沃茨。

The translocation of CO2 and chloride ions through gills will be studied using isolated perfused crab gills. CO2 flux across the gill is a process which can be complex due to several factors: 1) carbonic anhydrase, which catalyzes the hydration and dehydration reactions of CO2, is located on the cell surface and inside the cell; 2) CO2 is generated endogenously by active metabolic processes; and 3) CO2 hydrated to bicarbonate plays a role in chloride uptake via C1-/HCO3-exchange. The quantitative importance and contribution of the above processes to the excretion of CO2 out of the animal and the uptake of chloride during low salinity stress is poorly understood. This proposal outlines experiments testing hypotheses which will elucidate the dual (CO2 and C1-) and related translocation processes. This study, which has general relevance to ion uptake processes in any metabolically active epithelium, is designed to determine the existence and location of C1-/HCO3-exchanger, determine the amount and the fate of the CO2 produced by the gill, measure the quantitative contribution of endogenously produced CO2 to C1- /HCO3-exchange, and determine the quantitative function of cell surface and intracellular carbonic anhydrase in CO2 excretion and chloride uptake. The results have importance to our understanding of the interplay between acid balance, respiration and ion regulation of aquatic animals in normal and polluted waters.