Evolutionary Biology of Prolactins and Somatotropins

催乳素和生长激素的进化生物学

• 批准号: 8802618
• 负责人: Charles Nicoll
• 金额: $ 7.6万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-15 至 1990-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8802618&HistoricalAwards=false
• 关键词: Evolutionary; Biology; Prolactins; Somatotropins

The research will determine which structural features of certain hormones can be related to their biological activities. The hormones to be studied are prolactin (PRL), and growth hormone (GH), and the related protein placental lactogen. Fragments of the hormones will prepared by chemical and enzymatic cleavage and they will be tested for receptor binding activity using membrane preparations from various organs. The same question will be addressed using a different approach. Segments of these hormones that are thought to be of functional significance will be detected using the techniques of site specific mutagenesis. In addition, the amino acids in such segments of one kind of hormone (e.g. a PRL) will be replaced by he amino acids in the same segment of the other kind of hormone (i.e. GH). The mutant molecules will then be tested for receptor binding and biological activities. These hormones are involved in both normal and abnormal growth. Information on their structural features will be of value in producing analogue that can block their action or that have enhanced biological activities.

这项研究将确定哪些结构特征的某些 激素可能与其生物活性有关。 的 待研究的激素是催乳素和生长激素 (GH)和相关蛋白胎盘催乳素。 的片段 激素将通过化学和酶促裂解制备， 将使用膜检测它们的受体结合活性， 各种器官的制剂。 同样的问题将是 用不同的方法解决。 这些激素的片段 被认为具有功能重要性的 使用位点特异性诱变技术检测。 在 此外，一种激素的这些片段中的氨基酸 (e.g. PRL）将被相同氨基酸中的氨基酸取代。 另一种激素（即GH）的片段。 突变 然后将测试分子的受体结合和生物学特性， 活动 这些激素参与了正常和 异常生长 关于其结构特征的信息将 在生产可以阻止其作用的类似物方面具有价值，或者 增强了生物活性。