Regulation of the micF Gene and Mechanism of Action of the Transcript as a Repressor

在过去的几年里，大量的小RNA 被发现和鉴定。 其中一些已经被 被发现是调节分子。 一种称为micF的小RNA RNA被假设为负调节 外膜蛋白F（ompF）在细胞水平的表达 在大肠杆菌中的翻译。 micF RNA作为一种免疫调节剂的作用机制 阻遏物分子将被阐明。 其动力学 将确定合成和衰变。 体内水平的 在多拷贝质粒上具有micF的细胞中ompF m RNA， 参考菌株和micF缺失菌株将 研究了 将尝试形成micF RNA - ompF mRNA的体外杂交及其特性将被表征。 小RNA分子是否与DNA互补， 某些mRNA序列可以抑制这些mRNA的翻译 现在很感兴趣 是否有一种微小的RNA 参与ompC合成的调节 MicF RNA可能被证明是其他调控RNA基因的原型 在真细菌中发挥作用，如果 情况就是这样。

Within the past few years a large number of small RNAs have been discovered and characterized. Several of these have been found to be regulatory molecules. One small RNA termed micF RNA has been hypothesized to negatively regulate the expressions of outer membrane protein F (ompF) at the level of translation in E coli. The mechanism faction of micF RNA as a repressor molecular will be elucidated. The kinetics of its systhesis and decay will be determined. The in vivo levels of ompF m RNA in cells having micF on a multicopy plasmid, in reference strains and in micF deletion strain will be investigated. Attempts will be made to form a micF RNA - ompF m RNA hybrid in vitro and its properties will be characterized. The question of whether small RNA molecules complementary fo certain mRNA sequences can repress translation of those mRNAs is of much current interest. Specifically is there a micC RNA that participates in the regulation of ompC synthesis? MicF RNA may prove to be a prototype of other regulatory RNA genes that function in eubacteria and it would indeed be exciting if this were the case.