The Conformation of Nucleic Acids

核酸的构象

• 批准号: 8803281
• 负责人: W. Johnson
• 金额: $ 36.92万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-11-01 至 1993-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8803281&HistoricalAwards=false
• 关键词: Conformation; Nucleic; Acids

Dr. Johnson is continuing his work on the determination of the secondary structure of biological polymers in solution using circular dichroism (CD) spectroscopy. CD is an excellent technique for monitoring the secondary structure of optically active biological molecules. It is very sensitive to the orientation of nearest neighbor chromophores and it can be used when these molecules are in solution. It is also planned to make extensive use of 2D NMR in the near future. The focus of this research is to solve structural problems of current interest which involve nucleic acids. A special CD spectrometer has been developed on which measurements in the vacuum ultraviolet region can be made. This region is rich in information about the structure and conformation of macromolecules. Dr. Johnson will also work on improving the theory of CD so that the spectrum of a macromolecule can be directly related to its structure and conformation.

约翰逊博士正在继续他的工作， 二级结构的生物聚合物在溶液中使用 圆二色性（CD）光谱。 CD是一个很好的 光学监测二级结构的技术 活性生物分子 这是非常敏感的 最近邻发色团的取向，并且它可以用于 当这些分子在溶液中时。 还计划 在不久的将来，2D NMR将得到广泛的应用。 本研究的重点是解决结构问题， 目前感兴趣的涉及核酸。 一张特别的CD 光谱仪已开发的测量在 可以制作真空紫外区。 该地区盛产 关于结构和构象的信息 大分子 约翰逊博士还将致力于改善 CD理论，这样一个大分子的光谱就可以 与其结构和构象直接相关。