Functions of the Subunits of the Chloroplast ATP Synthase

叶绿体 ATP 合酶亚基的功能

• 批准号: 8803608
• 负责人: Richard Mc Carty
• 金额: $ 26.82万
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-11-15 至 1992-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8803608&HistoricalAwards=false
• 关键词: Functions; Subunits; Chloroplast; ATP; Synthase

The mechanism of proton-linked ATP synthesis by chloroplast, mitochondrial and bacterial membranes is a major unsolved problem in biochemistry. This award is aimed at determining whether the unusual asymmetry of this enzyme is altered by catalytic turnover. The roles of the delta subunit of the enzyme and its position in the macromolecule will be elucidated. Interaction between the gamma and epsilon subunits will be further investigated since these polypeptides play critical roles in the regulation of the enzyme's activity. Structural changes in the enzyme are induced by the formation of electrochemical proton gradients across the thylakoid membrane. Attempts to map these changes and to follow them kinetically will be made. This research will provide vital information to help us understand how energy is stored in living cells by the synthesis of an energy rich compound, adenosine triphosphate (ATP).

叶绿体质子连接ATP合成的机制， 线粒体和细菌膜是一个主要的未解决的 生物化学的问题 该奖项旨在确定 这种酶不寻常的不对称性是否被 催化周转 δ亚基的作用 酶及其在大分子中的位置将是 阐明。 γ亚基和γ亚基之间的相互作用 将进一步研究，因为这些多肽发挥 在调节酶活性方面发挥着关键作用。 酶的结构变化是由 电化学质子梯度穿过类囊体 膜的 试图描绘这些变化并跟踪它们 动力学将被制造出来。 这项研究将提供重要信息，帮助我们 了解能量是如何储存在活细胞中的 富含能量化合物三磷酸腺苷合成 （ATP）中指定。