Functions of the Subunits of the Chloroplast ATP Synthase

叶绿体 ATP 合酶亚基的功能

• 批准号: 9104742
• 负责人: Richard Mc Carty
• 金额: $ 32.1万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-12-01 至 1995-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9104742&HistoricalAwards=false
• 关键词: Functions; Subunits; Chloroplast; ATP; Synthase

The structure, mechanism and regulation of the ATP synthases of chloroplast, mitochondrial, and bacterial membranes are major problems in biochemistry. This proposal deals with chloroplast purification of the subunits of CFo, the integral membrane portion of the chloroplast ATP synthase, in active form, and the reconstituted of proton translocation and the binding of CF 1, the extrinsic, catalytic part of the synthase. Fluorescence resonance energy transfer will be used to extend the extensive structural map obtained for CF1 to CFo. The binding of nucleotides to CF1 in relation to initial rates of catalysis will be examined. Calorimetric analyses are proposed that should give information about ATP-induced conformational changes in CF1 as well as about the role of nucleotide binding in stabilizing the enzyme. Structural mapping of a fourth nucleotide binding site will be carried out. The role of the epsilon subunit of CF1 will be examined. The binding of epsilon to CF1 deficient in this subunit will be studied by fluorescence and by isothermal titration calorimetry.

ATP酶的结构、作用机制及调控 叶绿体、线粒体和细菌的膜是主要的 生物化学的问题 这个建议涉及叶绿体 CFo亚基的纯化， 叶绿体ATP合酶，在活性形式，和 重组的质子易位和CF 1的结合， 合成酶的外在催化部分。 荧光共振 能量转移将用于扩展广泛的结构图 从CF1到CFo。 核苷酸与CF1的结合， 将检查与催化的初始速率的关系。 建议进行量热分析， 关于ATP诱导的CF1构象变化以及关于 核苷酸结合在稳定酶中的作用。 第四个核苷酸结合位点的结构作图将是 贯彻 CF1的α亚基的作用将是 考察 在该亚基中，CF1与CF3的结合缺乏 将通过荧光和等温滴定法进行研究 量热法