Physical Chemical Basis of Control of Gene Expression

控制基因表达的物理化学基础

• 批准号: 8803673
• 负责人: M. Thomas Record
• 金额: $ 62.58万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8803673&HistoricalAwards=false
• 关键词: Physical; Chemical; Basis; Control; Gene

This project is in the Chemistry of Life Processes Initiative in the Office of Special Projects in Chemistry. The objectives of this research are to understand the physical chemical foundations of control of gene expression in living systems. The general thermodynamic principles governing stability and specificity of regulatory proteins and nucleic acids, and the important steps involved in the association and dissociation of these macromolecular species, are now reasonably understood in dilute solution under laboratory conditions. This project will extend the this level of chemical understanding to the crowded environment of the cell cytoplasm. This fundamental research will have biomedical and biotechnological significance. Experiments will quantify a) the chemical environment of the cytoplasm of E. coli and the variability of this environment, b) the properties of and structures of DNA in this environment, and c) the effects of the cytoplasmic environment on noncovalent interactions of proteins and nucleic acids. In particular the project wil address the origins of the in vivo-in vitro paradox in which protein-DNA interactions are highly salt dependent in vitro and salt independent in vivo. This project is a renewal of one previously supported by the Foundation.

这个项目是在生命过程的化学倡议 在化学特别项目办公室工作 目标 是为了了解 控制生命系统中基因表达的基础。 控制稳定性的一般热力学原理和 调节蛋白和核酸的特异性，以及 参与结合和解离的重要步骤 这些大分子物质，现在被合理地理解为 实验室条件下的稀溶液。 该项目将 将这种化学理解水平扩展到拥挤的 细胞质的环境。 这项基础研究 将具有生物医学和生物技术意义。 实验将量化a）化学环境的 E.大肠杆菌和这种环境的可变性， B）在这种环境中DNA的性质和结构， 和c）细胞质环境对 蛋白质和核酸的非共价相互作用。 在 特别是该项目将解决的起源， 体内-体外矛盾，其中蛋白质-DNA相互作用 在体外高度盐依赖性和在体内盐非依赖性。 该项目是以前由 基金会