Affinity Labeling of Purine Nucleotide Sites in Proteins

蛋白质中嘌呤核苷酸位点的亲和标记

• 批准号: 8804706
• 负责人: Roberta Colman
• 金额: $ 28.02万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-15 至 1991-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8804706&HistoricalAwards=false
• 关键词: Affinity; Labeling; Purine; Nucleotide; Sites

The goal of this award is to develop the tools which will allow a systematic exploration of the amino acid residues in the purine nucleotide binding sites of proteins. Such sites will be affinity labeled using reagents which contain alkylating groups capable of reacting covalently with the protein. The purine nucleotide moiety will thus provide the specifity for chemical modification of the proteins within the binding sites. The effects of these reagents will be examined on three proteins, pyruvate kinase, a membrane-bound ADP receptor protein from human platelets and cAMP phosphodiesterase. The results of the research should enhance our understanding of the structure and architecture of the active sites of proteins, and how these biomacromolecules catalyze or regulate the myriad reactions that occur in living systems.

该奖项的目标是开发一种工具，可以系统地探索蛋白质嘌呤核苷酸结合位点上的氨基酸残基。这些位点将使用含有能够与蛋白质共价反应的烷基化基团的试剂进行亲和标记。嘌呤核苷酸片段因此将为结合位点内蛋白质的化学修饰提供特异性。这些试剂对三种蛋白质的影响将被检验，丙酮酸激酶，一种来自人血小板的膜结合ADP受体蛋白和cAMP磷酸二酯酶。该研究结果将增强我们对蛋白质活性位点的结构和结构的理解，以及这些生物大分子如何催化或调节生命系统中发生的无数反应。