Development of Ligand-tethered Cu-responsive protein labeling chemical tool for visualizing Cu-release at synaptic cleft

开发配体束缚的铜响应蛋白标记化学工具，用于可视化突触间隙铜的释放

• 批准号: 22KJ1889
• 负责人: CHENG RONG
• 金额: $ 1.09万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KJ1889/
• 关键词: biometal; copper ion; cuprous ion; protein labeling; conditional proteomics; chemical proteomics

This year, several cuprous-ion-responsive protein labeling reagents (CuRs) have been synthesized, and the chemical structure has been optimized. The cuprous ions-responsive reactions of the optimal CuR have also been studied and validated by HPLC analysis. Moreover, the capability of CuR to label and profile proteins in response to cuprous ions in multiple living cell systems has also been confirmed by western blotting, imaging, and proteomics analysis.

今年，已经合成了几种亚铜离子响应蛋白质标记试剂（CuRs），并对其化学结构进行了优化。最佳CuR的亚铜离子响应反应也进行了研究，并通过HPLC分析验证。此外，CuR在多个活细胞系统中响应亚铜离子标记和分析蛋白质的能力也已通过蛋白质印迹、成像和蛋白质组学分析得到证实。

项目成果

Development of selective Cu+-responsive protein labeling reagent towards conditional proteomics

条件蛋白质组学选择性铜响应蛋白标记试剂的开发

• 发表时间: 2022
• 作者: Rong Cheng.;Tomonori Tamura.;Itaru Hamachi
• 通讯作者: Itaru Hamachi