Development of Ligand-tethered Cu-responsive protein labeling chemical tool for visualizing Cu-release at synaptic cleft

今年合成了几种铜离子反应蛋白标记试剂，并对其化学结构进行了优化。并通过高效液相色谱法对最优CuR的铜离子反应进行了研究和验证。此外，western blotting、成像和蛋白质组学分析也证实了CuR在多种活细胞系统中对铜离子的反应进行标记和分析的能力。

This year, several cuprous-ion-responsive protein labeling reagents (CuRs) have been synthesized, and the chemical structure has been optimized. The cuprous ions-responsive reactions of the optimal CuR have also been studied and validated by HPLC analysis. Moreover, the capability of CuR to label and profile proteins in response to cuprous ions in multiple living cell systems has also been confirmed by western blotting, imaging, and proteomics analysis.