RUI: ATP Regeneration Based on Immobilized Polyphosphate Kinase

这项建议的目的是发展一种固定的 使用多磷酸激酶再生ATP的酶系统 从大肠杆菌。 目标有四个方面。 第一 就是提高酶的成本效益 通过提高其速度，能力和 产率 第二种是将多磷酸激酶 不同的不溶性支持物，以确定最可行的 酶结合物。 三是构筑填充床 反应器，并确定性能参数， 连续流系统。 第四个目标是确定 动力学的最佳操作参数， 再生过程 这项工作提供了一个极好的机会， 大学生获得实践经验的发展 一个可能在经济上变得重要的过程， 生物技术操作。

The purpose of this proposal is to develop an immobilized enzyme system for ATP regeneration using polyphosphate kinase from Escherichia coli. The objectives are fourfold. The first is to increase the cost-effectiveness of the enzyme purification process by improving its speed, capacity and yield. The second is to immobilize polyphosphate kinase on different insoluble supports so as to identify the most viable enzyme conjugates. The third is to construct packed bed reactors and determine the performance parameters in continuous-flow systems. The fourth objective is to determine kinetically the optimal operating parameters for the regeneration process. The work provides an excellent opportunity for undergraduates to gain hands on experience in the development of a process that may become economically important in biotechnological operations.