MALDI-TOF Mass Spectrometry in Chemistry and Biochemistry

化学和生物化学中的 MALDI-TOF 质谱分析

• 批准号: 0126950
• 负责人: Owen Moe
• 金额: $ 3.95万
• 依托单位: Lebanon Valley College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0126950&HistoricalAwards=false
• 关键词: MALDI; TOF; Mass; Spectrometry; Chemistry

Chemistry (12) The technique, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry has become a foundational tool in chemical and biochemical research. Using MALDI-TOF one can quickly and accurately determine masses of proteins, nucleic acids, and other polymers well in excess of 100,000 and, just as importantly, resolve and analyze complex mixtures of smaller peptides and oligonucleotides. We are introducing this powerful new technique into the undergraduate curriculum by implementing MALDI-based experiments in five laboratory courses and by incorporating MALDI in ongoing student-faculty research projects. Our goal is to have students gain experience with MALDI-TOF technique and develop an appreciation for the applicability of the technique to a wide range of problems. In the instrumental analysis course students are using the MALDI-TOF technique to identify unknown proteins through peptide mapping, they synthesize polystyrene in organic chemistry and determine its molecular mass distribution, they restriction map a multiple cloning site in molecular biology, they create a profile of cysteine residues for a protein in biochemistry, and they identify unknown bacteria in microbiology. These new experiments, adapted from the educational and scientific literature, are enhancing the laboratory experience of our students by involving them in the cutting-edge mass spectroscopic tools needed to address many challenging problems encountered in the chemical and biochemical research laboratories.

化学 (12) 基质辅助激光解吸电离飞行时间 (MALDI-TOF) 质谱技术已成为化学和生化研究的基础工具。使用 MALDI-TOF，人们可以快速准确地测定质量超过 100,000 的蛋白质、核酸和其他聚合物，同样重要的是，可以解析和分析较小肽和寡核苷酸的复杂混合物。我们通过在五门实验室课程中实施基于 MALDI 的实验，并将 MALDI 纳入正在进行的师生研究项目，将这种强大的新技术引入本科课程。我们的目标是让学生获得 MALDI-TOF 技术的经验，并了解该技术对各种问题的适用性。在仪器分析课程中，学生使用MALDI-TOF技术通过肽图谱识别未知蛋白质，在有机化学中合成聚苯乙烯并确定其分子量分布，在分子生物学中绘制多克隆位点限制性图谱，在生物化学中创建蛋白质的半胱氨酸残基图谱，并在微生物学中识别未知细菌。这些新实验改编自教育和科学文献，通过让我们的学生参与解决化学和生化研究实验室中遇到的许多挑战性问题所需的尖端质谱工具，增强了学生的实验室体验。