Electron Flow in Photosynthetic Bacteria

光合细菌中的电子流

• 批准号: 8806609
• 负责人: David Knaff
• 金额: $ 17.88万
• 依托单位: Texas Tech University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8806609&HistoricalAwards=false
• 关键词: Electron; Flow; Photosynthetic; Bacteria

The specific objectives of this project are (1) to characterizethe cytochrome bc1 complex of a photosynthetic purple non-sulfur bacterium by resonance Raman spectroscopy and to characterize the binding sites on the complex for quinone, cytochrome c2 and the inhibitor antimycin A and (2) to compare the cytochrome bc1 complex and its soluble cytochrome c electron acceptor in apurple sulfur bacterium with those of purple non-sulfur bacteria. Also to compare the spectroscopic properties of these cytochrome bc1 complexes with the functionally analogous chloroplast cytochrome b6f complex. Emphasis is placed on unique aspects of bioenergetics in these bacteria or on the simplicity of processes in these bacteria compared to the analogous one in mitochondrial respiration. A number of spectroscopic (e.g., resonance Raman and electron paramagnetic resonance spectroscopy), biochemical (e.g., chemical modification of specific amino acids and photoaffinity labeling) and genetic techniques will be utilized in an attempt to provide details at the molecular level about these electron transfer reactions that are central to energy transduction in photosynthetic bacteria. %%% The energy needed for cell metabolism is generated through several different mechanisms. In this study, electron transport linked to an electrochemical gradient will be studied in a photosynthetic bacterium. This is a convenient experimental system because electron transfer can be started and stopped by turning light on or off.

The specific objectives of this project are (1) to characterizethe cytochrome bc1 complex of a photosynthetic purple non-sulfur bacterium by resonance Raman spectroscopy and to characterize the binding sites on the complex for quinone, cytochrome c2 and the inhibitor antimycin A and (2) to compare the cytochrome bc1 complex and its soluble cytochrome c electron紫水硫细菌中的受体和紫色非硫细菌的受体。还要比较这些细胞色素BC1复合物与功能类似的叶绿体细胞色素B6F复合物的光谱特性。 与线粒体呼吸中的类似性相比，重点放在这些细菌中生物能学的独特方面或这些细菌过程的简单方面。 A number of spectroscopic (e.g., resonance Raman and electron paramagnetic resonance spectroscopy), biochemical (e.g., chemical modification of specific amino acids and photoaffinity labeling) and genetic techniques will be utilized in an attempt to provide details at the molecular level about these electron transfer reactions that are central to energy transduction in photosynthetic bacteria. %%通过几种不同的机制产生细胞代谢所需的能量。 在这项研究中，将在光合细菌中研究与电化学梯度相关的电子传输。 这是一个方便的实验系统，因为可以通过打开或关闭照明来启动和停止电子传输。