Chemistry Structure and Function of the Non-Tubulin Spindle Matrix in Dividing Cells

分裂细胞中非微管蛋白纺锤体基质的化学结构和功能

• 批准号: 8811015
• 负责人: Richard Linck
• 金额: $ 11.54万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8811015&HistoricalAwards=false
• 关键词: Chemistry; Structure; Function; Non; Tubulin

This proposal deals with the study of the mitotic spindles of invertebrates zygotes. The major goal is to purify tektin-like proteins from mitotic spindles and to determine how these proteins interact with the microtubules of the spindle and the spindle matrix. Tektin antibodies will be used to identify and purify similar proteins from the spindles and these proteins will be used to generate antibodies to be used as specific probes. Laser confocal light microscopy and transmission and scanning electron microscopy will be used to determine the 3-dimensional organization of these matrix polypeptides. Antibodies will be microinjected into living cells to determine effects on spindle structure and function. These studies will advance our understanding of microtubule assembly, polarity and stability in the mitotic spindle and how the matrix provides a framework for the operation of the cell division process.

该提案涉及无脊椎动物受精卵有丝分裂纺锤体的研究。 主要目标是从有丝分裂纺锤体中纯化 tektin 样蛋白，并确定这些蛋白如何与纺锤体微管和纺锤体基质相互作用。 Tektin 抗体将用于识别和纯化纺锤体中的类似蛋白质，这些蛋白质将用于生成用作特定探针的抗体。 激光共焦光学显微镜以及透射和扫描电子显微镜将用于确定这些基质多肽的3维组织。 抗体将被显微注射到活细胞中，以确定对纺锤体结构和功能的影响。 这些研究将增进我们对有丝分裂纺锤体的微管组装、极性和稳定性以及基质如何为细胞分裂过程的运行提供框架的理解。