Surface Potentials and Second Messengers

本项目的目的是检查静电 生产第二信使的潜力。 的 磷脂酰肌醇4，5-二磷酸（PIP 2）水解， 脂质位于细胞膜的细胞质表面， 产生肌醇1，4，5-三磷酸（IP 3）和甘油二酯 （DG）。 IP 3诱导钙的释放; DG激活蛋白质 激酶C McLaughlin博士将开展一系列项目 包括：1）研究多价阳离子的结合，如 2）标记PIP 2的烃尾，和 进行荧光能量转移测量; 3）研究 静电势对钙离子能力的影响 激活聚磷酸肌醇特异性磷脂酶C;和4） 继续进行理论和实验研究， 多价离子结合中的表面势和协同性 在双层膜中将阳离子转化为单价阴离子脂质。 尽管很少有生物药理学家在这一领域工作， 关于物理因素的信息很少， 影响这些第二种物质的产生和作用机制 使者们，有许多证据， 相信这些因素之一是静电 由带电脂质如磷脂酰丝氨酸产生的电势。

The objective of this project is to examine the electrostatic potentials on the production of second messengers. The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), a lipid located on the cytoplasmic surface of cell membranes, produces inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DG). IP3 induces the release of calcium; DG activates a protein kinase C. Dr. McLaughlin will carry out a number of projects including: 1) studying the binding of polyvalent cations like neomycin to PIP2; 2) labelling the hydrocarbon tails of PIP2 and making fluorescence energy transfer measurements; 3) studying the effect of electrostatic potentials on the ability of calcium to activate a polyphosphoinositide-specific phospholipase C; and 4) continuing theoretical and experimental studies of the role of surface potentials and cooperativity in the binding of polyvalent cations to monovalent anionic lipids in bilayer membranes. Although very few biophysicists are working in this area and little information is available about the physical factors that influence the production and mechanism of action of these second messengers, there is a great deal of evidence present for believing that one of these factors is the electrostatic potential produced by charged lipids such as phosphatidylserine.