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U.S.-Italy Cooperative Research: Structure and Function of Glutamate Synthase

美意合作研究：谷氨酸合成酶的结构和功能

基本信息

批准号：
8815289
负责人：
Dale Edmondson
金额：
$ 1.28万
依托单位：
Emory University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1989
资助国家：
美国
起止时间：
1989-02-01 至 1993-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=8815289&HistoricalAwards=false
关键词：
U.S.Italy Cooperative Research Structure

项目摘要

This award will support Prof. Dale Edmondson of Emory University to engage in collaborative activities with Dr. Bruno Curti and his group at the University of Milan. The researchers will focus on the enzyme glutamate synthase, which plays a key role in the assimilation of nitrogen in nitrogen-fixing microorganisms. Specifically, they will pursue the investigation of the roles of the flavin and iron-sulfur centers in the catalytic mechanisms of glutamate synthase isolated from two nitrogen-fixing bacteria, Azospirillum brasilense and Azotobacter vinelandii, the first of which is microaerophilic and associated with plant roots while the second is an aerobic free-living soil bacterium. Experimental work on the enzymes will be conducted at both Emory and Milan. Azospirillum glutamate synthase will be purified and characterized in Milan while the Azotobacter enzyme will be purified in Atlanta. The U.S. lab will provide the expertise in physical measurements on the enzyme systems. The interaction between Dr. Edmondson, who has an extensive background in areas of biophysical chemistry in general and flavoproteins in particular, and Dr. Curti's group, which has worked with the enzyme glutamate synthase for several years, should be of mutual benefit. Furthermore, it has the potential for yielding substantial results concerning the basic structure and mechanism of glutamate synthase as well as new information concerning nitrogen metabolism.

该奖项将支持埃默里大学的 Dale Edmondson 教授与米兰大学的 Bruno Curti 博士及其团队开展合作活动。研究人员将重点关注谷氨酸合酶，它在固氮微生物的氮同化中发挥着关键作用。具体来说，他们将研究黄素和铁硫中心在谷氨酸合酶催化机制中的作用，该酶从两种固氮细菌（巴西固氮螺菌和维氏固氮菌）中分离出来，第一种是微需氧的，与植物根部相关，第二种是需氧的自由生活土壤细菌。酶的实验工作将在埃默里大学和米兰大学进行。固氮螺菌谷氨酸合酶将在米兰进行纯化和表征，而固氮杆菌酶将在亚特兰大进行纯化。美国实验室将提供酶系统物理测量的专业知识。埃德蒙森博士在生物物理化学领域，尤其是黄素蛋白领域拥有广泛的背景，而柯蒂博士的团队多年来一直致力于谷氨酸合酶的研究，他的团队之间的互动应该是互惠互利的。此外，它有可能在谷氨酸合酶的基本结构和机制以及氮代谢的新信息方面产生实质性结果。