RUI: Characterization of the Naphthylphthalamic Acid (NPA)-Inhibitable Auxin Anion Carrier Protein

本项目的目标是分离和表征 植物干细胞膜中参与运输的蛋白质 以及植物生长调节剂生长素的作用。 生长素合成 并通过干细胞运输 而不是由循环系统携带。 运送 以单向方式通过这些细胞，从顶点到 基地 这种运输是生长素刺激植物生长所必需的。 延长这些细胞。 萘酞酰胺酸（NPA），一种 除草剂，特异性地与细胞膜中的蛋白质结合， 抑制生长素运输。这种NPA结合蛋白， 大部分位于干细胞膜的下端，被认为是 参与生长素的单向运输。建立结构 了解这种蛋白质在生长素运输中的作用将有助于 在理解这种运输过程的机制， 了解生长素控制的协调生长和发育 植物。 该提案将使用NPA的放射性类似物，5 '-叠氮基-NPA（Az-100）。 NPA）以放射性标记NPA结合蛋白，纯化NPA结合蛋白。 放射性标记的蛋白质，并确定的氨基酸序列 分离的蛋白质的氨基末端部分。

The objectives of this project are to isolate and characterize a protein in plant stem cell membranes that is involved in the transport and action of the plant growth regulator, auxin. Auxin is synthesized in the growing tips of plants and is transported through stem cells rather than being carried by a circulatory system. It is transported through these cells in a unidirectional fashion, from the apex to the base. This transport is required for auxin stimulation of the elongation of these cells. Naphthylphthalamic Acid (NPA), an herbicide, binds specifically to a protein in cell membranes, inhibiting auxin transport. This NPA-binding protein, which is located largely on the lower ends of stem cell membranes, is thought to be involved in unidirectional auxin transport. Establishing the structure of this protein and understanding its role in auxin transport will aid in understanding the mechanism of this transport process and in understanding the auxin-controlled coordinated growth and development of plants. This proposal will use a radioactive analog of NPA, 5'-azido-NPA (Az- NPA) to radioactively label the NPA-binding protein, purify the radioactively labeled protein, and determine the amino acid sequence of the amino terminal portion of the isolated protein.