Crystallographic Studies of p-Cresol Methylhydroxylase and Methylamine Dehydrogenase Enzyme Systems

对甲酚甲基羟化酶和甲胺脱氢酶系统的晶体学研究

• 批准号: 8816618
• 负责人: F. Mathews
• 金额: $ 30.6万
• 依托单位: Washington University School of Medicine
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8816618&HistoricalAwards=false
• 关键词: Crystallographic; Studies; Cresol; Methylhydroxylase; Methylamine

X-ray diffraction studies will be carried out on two bacterial enzyme systems each involving oxidation-reduction and electron transfer reactions. An electron density map for crystals of p-cresol methylhydroxylase has been computed at 3.0 A resolution and the paths of the polypeptide chains have been traced. After the amino acid sequence has been fitted to the density, the structure will be refined and the binding of substrate and inhibitors studied. Crystals of amicyanin, methylamine dehydrogenase and of their intermolecular complex have been prepared. Their structures will be analyzed by anomalous scattering, multiple isomorphous replacement and/or molecular replacement methods Dr. Mathews has engaged in structural studies of biological macromolecules using X-ray diffraction methods for many years. His work on b type cytochromes has led in part to a working hypothesis of how electron transport proteins interact and pass electrons from one to another. His approach is unique in that one of the systems under study has both the donor and acceptor electron transfer groups in the same molecule, while the other system contains a novel prosthetic group.***//

将对两种细菌进行X射线衍射研究。 涉及氧化还原和电子的酶系统 转移反应 晶体的电子密度图 对甲酚甲基羟化酶的计算分辨率为3.0 并追踪了多肽链的路径。 后 氨基酸序列已被拟合到密度， 结构将得到改善，并结合基板和 研究的抑制剂。阿米花青、甲胺的晶体 脱氢酶和它们的分子间复合物已经被 制备 它们的结构将通过反常的 散射、多个同晶置换和/或分子 替换方法 马修斯博士从事生物结构研究 使用X射线衍射方法研究大分子已经很多年了。 他在B型细胞色素方面的工作， 电子传递蛋白如何相互作用和传递的假说 电子从一个到另一个。 他的方法是独一无二的， 研究中的一个系统同时具有供体和受体， 电子转移基团在同一分子中，而另一个 系统包含一个新的辅基。*//