Collaborative Research in Maize Genetics

该奖项授予伊利诺伊州立大学，将资助韦伯博士有效参与与密苏里大学David Hoisington和Ed Coe的合作研究项目所需的小型设备项目（NSF 8721921）。该项目将继续并扩大合作研究，旨在绘制玉米的综合物理和遗传图谱。几种类型的遗传作图策略将用于识别和关联RFLP标记、细胞学标记（凸起、缺失、插入和倒置）、生化标记（同工酶的突变）和其他常规标记的位置。尽管这些不同类型的标记物的位置信息已经有了相当大的数量，但是在细胞学标记物图谱与生物化学标记物图谱、RFLP图谱与细胞学图谱等方面的关联研究相对较少。由于RFLP图谱尚不完整，也因为没有一个实验室能够组装出一套完整的RFLP标记dna供所有研究人员使用，所以我们将重点放在新开发的RFLP图谱技术上。虽然这项研究将主要由dr。Coe和Hoisington需要与其他玉米遗传学家合作，特别是那些熟练识别生化标记的人。因此，伊利诺伊州立大学以及爱荷华州立大学和北卡罗来纳州立大学的其他研究者同时获得了小额奖励。这些奖金将提供实验室设备和计算机，这些设备和计算机将有助于分析和比较在独立实验室中开发的数据，并将确保每个研究者开发的研究材料将易于所有合作者使用。传统上，遗传作图技术一直是对各种生物学基本问题感兴趣的研究人员以及植物和动物育种者的有用工具。最近，一项名为RFLP图谱的强大新技术的发展，将对遗传学在解决基础问题和应用问题上的轻松程度产生重大影响。然而，重要的是要保留更传统技术的知识和工具，并将它们与新的RFLP地图集成（或相关）。玉米的情况尤其如此，它一直是广泛遗传研究的主题。拟议的合作研究将加速完整的玉米RFLP地图的开发，并将确保该地图是所有研究人员和农学家可用的工具。此外，通过促进RFLP数据与其他类型遗传标记获得的数据的整合，该研究将确保RFLP图谱对那些需要它的人具有最大的效用。

This award to Illinois State University will fund the purchase of small equipment items necessary for the effective participation of Dr. Weber in a collaborative research project with David Hoisington and Ed Coe at the University of Missouri (NSF 8721921). The project will continue and expand collaborative research aimed at producing an integrated physical and genetic map of maize. Genetic mapping strategies of several types will be used to identify and correlate the positions of RFLP markers, cytological markers (knobs, deletions, insertions and inversions), biochemical markers (mutations responsible for isozymes) and other, conventional markers. Although a substantial amount of positional information for these various types of markers is now available, relatively little has been done to correlate the map of cytological markers with that of the biochemical ones, the RFLP map with the cytological map and so on. Particular focus is placed on the newly developed technique of RFLP mapping because the map is not yet complete, and because no single lab has yet assembled a complete set of RFLP marker DNAs that are available to all researchers. Although the research will be performed principally by Drs. Coe and Hoisington, collaboration with other corn geneticists, especially those skilled in the identification of biochemical markers, is required. Thus, small concurrent awards are being made to this investigator at Illinois State, as well as others at both Iowa State and North Carolina State Universities. These awards will provide laboratory equipment and computers that will facilitate analysis and comparison of data developed in the separate labs and will insure that research materials developed by each investigator will be readily accessible to all the collaborators. Traditionally, techniques for genetic mapping have been a useful tool for researchers interested in a variety of basic questions in biology as well as for plant and animal breeders. The recent development of a powerful new technique called RFLP mapping will have a significant effect on the ease with which genetics can be brought to bear on both basic and applied problems. However, it is important that the knowledge and tools of the more traditional techniques be preserved and that they be integrated (or correlated) with the new RFLP maps. This is particularly true in the case of corn, which has been the subject of extensive genetic study. The proposed collaborative research will accelerate the development of a complete RFLP map of corn and will insure that the map is a tool available to all researchers and agronomists. Moreover, by facilitating the integration of the RFLP data with that obtained with other types of genetic markers, the research will insure that the RFLP map is of maximum utility for those who need it.